ADAM12 antibody - Aminoterminal end catalytic domain (ab28225)

Product Name 

ADAM12 antibody - Aminoterminal end catalytic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAM12 - Aminoterminal end catalytic domain

Immunogen 

Synthetic peptide based on the amino end of the catalytic domain of human ADAM 12.

(Peptide available as ab41205.)

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat
(Due to sequence homology)

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all ADAM12 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. ab28225 recognizes bands of 116 kDa (minor band), 92 kDa (major band), and cleaved products at 68 kDa, 40 kDa in reduced Western blots of cell lysates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Type I membrane protein (isoform 12L). A secreted form (isoform 12S) is produced by alternative splicing.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha

Relevance 

ADAM12 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. Two forms of ADAM12 have been described: ADAM12S and ADAM12L (short and long forms). The short form is a soluble form lacking the transmembrane and cytoplasmic domains. The short form of ADAM12 was reported to provoke myogenesis, and the lack of cytoplasmic domain suggests different regulation pathways (although both forms can be expressed in the same tissue). Other papers investigated the SH3 ligand domains in the cytoplasmic portion of ADAM12, demonstrating regulation routes for ADAM12 via Src and Src tyrosine kinase. ADAM12 is primarily expressed in muscle and bone (although placenta, osteoblasts, and many tumor cell lines also express ADAM12). ADAM12 supports cell adhesion by acting as a cell attachment molecule and binding integrins through the cysteine rich domain. A marker of skeletal muscle regeneration, it has been implicated in myoblast differentiation and fusion. ADAM12 contains the canonical HExxHxxxxxH zinc metalloproteinase motif. Full length ADAM12 (909 amino acids) has a predicted mass of 99.5 kDa, but due to glycosylation and cyteine rich regions, the reduced protein migrates to 114 kDa (unprocessed) and 84 kDa (processed). The soluble form of ADAM12 (738 amino acids, predicted mass of 80.5 kDa) is seen as a 92 kDa zymogen and 68 kDa furin processed form.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for ADAM12 antibody - Aminoterminal end catalytic domain images, references, products related to ab28225 and other tools.

ADAM12 antibody - Aminoterminal end catalytic domain images:

References for ADAM12 antibody - Aminoterminal end catalytic domain (ab28225)

  This product has been used in: (two most recent references)

Estrella C et al. Role of A Disintegrin and Metalloprotease-12 in Neutrophil Recruitment Induced by Airway Epithelium. Am J Respir Cell Mol Biol : (2009). PubMed: 19213876

Zhang L et al. Adam12 plays a role during uterine decidualization in mice. Cell Tissue Res : (2009). WB; Mouse. PubMed: 19841944
If you publish research using ab28225 please let us know so that we can cite the reference on this datasheet.

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