Overview

  • Product nameAnti-ADAM13 antibodySee all ADAM13 primary antibodies ...
  • Description
    Rabbit polyclonal to ADAM13
  • Specificityab39157 recognises metalloproteinase ADAM-13.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Xenopus laevis
  • Immunogen

    Synthetic peptide based on the amino end of Xenopus ADAM13. (Peptide available as ab41208.)

Properties

Applications

Our Abpromise guarantee covers the use of ab39157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. The antibody recognizes bands of 120 kDa (minor band), 92 kDa (major band), and cleaved products at 68 kDa, 40 kDa in reduced Western blots of cell lysates. (Predicted molecular weight: 100 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceADAM13 was first described as a protein expressed in somatic mesoderm and neural crest cells, in developing Xenopus embryos. ADAM13 was also found in liver, heart, and intestines from adult Xenopus. ADAM13 may regulate cellular signaling via Src and Src tyrosine kinase. ADAM13 may also act as a cell attachment molecule, by binding integrins through the cysteine rich domain amoung many other roles. A member of the metalloproteinase family containing disintegrin like domains (ADAMs) the functions of ADAM13 are still poorly understood. ADAM13 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, as well as disintegrin, cysteine rich, EFG like, transmembrane and Cytoplasmic domains. ADAM13 has been shown to be proteolytically active, cleaving fibronectin after binding it to the EGF like domain. ADAM13 is also shed from cells in culture, cleaved aminoterminal from the transmembrane domain, and is released into the culture media. Shed ADAM13 is a 52 kD protein, and can form complexes with a2 macroglobulin, suggesting it is a competent protease. Xenopus ADAM13 has greatest homology with human ADAM 33 (51% identical), and is 46% identical with human or mouse ADAM12 or ADAM19. It is still unclear if any of these ADAMs are species orthologs of Xenopus ADAM13, but there are significant differences between the related sequences, suggesting that ADAM13 may be a unique protein. The full length Xenopus ADAM13 sequence codes for a 914 amino acid protein. Predicted mass is 99.749 kD, but glycosylation and cyteine rich regions give Xenopus ADAM13 an apparent MW of 120 kD unprocessed, and 97 kD processed forms, on reduced SDS PAGE gels. ADAM13 contains a putative furin cleavage site, suggesting that a prohormone convertase cleaves the propeptide domain away from the catalytic domain.
    • Database links
      • Alternative names
        • A Disintegrin And Metalloproteinase 13 antibody
        • A Disintegrin And Metalloproteinase 13 antibody
        • XADAM13 antibody
        • XADAM13 antibody

      References for Anti-ADAM13 antibody (ab39157)

      ab39157 has not yet been referenced specifically in any publications.

      Product Wall

      The ab39157 epitope is within the propeptide domain of Xenopus ADAM-13. The propeptide domain runs from residues 34-155. The epitope for the ab39158 is at the carboxyterminal end of the molecule. I hope this is helpful. Please contact me again ...

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      The ab39157 antibody epitope is within the propeptide domain, which is defined as residues 30-74 of the human sequence. The antibody recognizes the latent form of ADAMTS-13, and the shed propeptide domain, but not the mature ADAMTS-13. ab39158 reacts t...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"