Overview

  • Product nameAnti-ADAM15 antibody [EPR5619]
    See all ADAM15 primary antibodies
  • Description
    Rabbit monoclonal [EPR5619] to ADAM15
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in the extracellular domain of Human ADAM15.

  • Positive control
    • SW480 cell lysate; Human colon tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab124698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90, 110 kDa (predicted molecular weight: 93 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionActive metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.
    • Tissue specificityExpressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level). Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells.
    • Sequence similaritiesContains 1 disintegrin domain.
      Contains 1 EGF-like domain.
      Contains 1 peptidase M12B domain.
    • DomainThe cytoplasmic domain interacts with endophilin I and sorting nexin 9.
      Disintegrin domain binds to integrin alphaV-beta3.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      The precursor is cleaved by a furin endopeptidase.
      Phosphorylation increases association with PTKs.
    • Cellular localizationEndomembrane system. Cell junction > adherens junction. Cell projection > cilium > flagellum. Cytoplasmic vesicle > secretory vesicle > acrosome. The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed.
    • Information by UniProt
    • Database links
    • Alternative names
      • A disintegrin and metalloproteinase domain 15 (metargidin) antibody
      • A disintegrin and metalloproteinase domain 15 antibody
      • ADA15_HUMAN antibody
      • ADAM 15 antibody
      • ADAM metallopeptidase domain 15 antibody
      • Adam15 antibody
      • and cysteine-rich protein 15 antibody
      • Disintegrin and metalloproteinase domain-containing protein 15 antibody
      • disintegrin-like antibody
      • EC 3.4.24. antibody
      • MDC 15 antibody
      • MDC-15 antibody
      • MDC15 antibody
      • Metalloprotease RGD disintegrin protein antibody
      • Metalloproteinase like disintegrin like and cysteine rich protein 15 antibody
      • Metalloproteinase-like antibody
      • Metargidin antibody
      see all

    Anti-ADAM15 antibody [EPR5619] images

    • Anti-ADAM15 antibody [EPR5619] (ab124698) at 1/1000 dilution + SW480 cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP at 2000

      Predicted band size : 93 kDa
    • ab124698, at 1/250 dilution, staining ADAM15 in paraffin-embedded Human colon tissue by immunohistochemistry..
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-ADAM15 antibody [EPR5619] (ab124698)

    ab124698 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"