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Our Abpromise guarantee covers the use of ab2051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 93 kDa.Can be blocked with ADAM17 peptide (ab7881). Detects bands of 80-130 kDa bands, which may represent mature protein, precursor, and glycosylated TACE.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 19553533
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC image of ADAM17 staining in Human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2051, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab2051 at 10µg/ml staining ADAM17 in Hela cells by ICC/IF
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