Overview

  • Product name
  • Description
    Rabbit polyclonal to ADAM17
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ADAM17 aa 807-823.
    Sequence:

    ASFKLQRQNRVDSKETE


    (Peptide available as ab7881)

  • Positive control
    • HeLa whole cell lysate, or Jurkat whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human pancreas adenocarcinoma.
  • General notes
    TNFa Converting Enzyme.

Properties

Applications

Our Abpromise guarantee covers the use of ab2051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 93 kDa.Can be blocked with ADAM17 peptide (ab7881). Detects bands of 80-130 kDa bands, which may represent mature protein, precursor, and glycosylated TACE.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 19553533

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
  • Tissue specificity
    Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
  • Sequence similarities
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • Domain
    Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
    Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
    • A disintegrin and metalloproteinase domain 17 antibody
    • ADA17_HUMAN antibody
    • ADAM 17 antibody
    • ADAM metallopeptidase domain 17 antibody
    • ADAM17 antibody
    • ADAM17 protein antibody
    • CD 156b antibody
    • CD156b antibody
    • CD156b antigen antibody
    • CSVP antibody
    • Disintegrin and metalloproteinase domain-containing protein 17 antibody
    • MGC71942 antibody
    • NISBD antibody
    • NISBD1 antibody
    • Snake venom like protease antibody
    • Snake venom-like protease antibody
    • TACE antibody
    • TNF alpha convertase antibody
    • TNF alpha converting enzyme antibody
    • TNF-alpha convertase antibody
    • TNF-alpha-converting enzyme antibody
    • Tumor Necrosis Factor Alpha Converting Enzyme antibody
    see all

Anti-ADAM17 antibody images



  • Predicted band size : 93 kDa
    Western Blot of HeLa (1, 4), Jurkat (2, 5) and Raji (3, 6) cell lysates labeling ADAM17 with Anti-ADAM17 antibody (ab2051) at 0.5µg/ml in the absence (1-3) or presence of blocking peptide (4-6).
  • All lanes : Anti-ADAM17 antibody (ab2051) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate with absence of blocking peptide
    Lane 2 : Jurkat whole cell lysate with absence of blocking peptide
    Lane 3 : HeLa whole cell lysate with ADAM17 peptide (ab7881)
    Lane 4 : Jurkat whole cell lysate with ADAM17 peptide (ab7881)


    Predicted band size : 93 kDa
    80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated ADAM17.
  • IHC image of ADAM17 staining in Human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2051, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunofluorescence of TACE in HeLa cells using ab2051 at 10 ug/ml.

  • Lane 1 : Anti-ADAM17 antibody (ab2051) at 0.5 µg/ml
    Lane 2 : Unrelated antibody of the same subclass as ab2051.

    Lane 1 : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cells
    Lane 2 : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cells

    Secondary
    HRP conjugated goat polyclonal antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 100,125 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab2051 at 10µg/ml staining ADAM17 in Hela cells by ICC/IF

  • All lanes : Anti-ADAM17 antibody (ab2051) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : HT1080 cell lysate
    Lane 4 : ES cell lysate
    Lane 5 : L929 cell lysate
    Lane 6 : CHO cell lysate
    Lane 7 : 293T cell lysate
    Lane 8 : ADAM17-overexpressing 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Rabbit polyclonal antibody at 1/4000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 93 kDa
    Additional bands at : 78 kDa (possible isoform).

    Exposure time : 15 seconds

    This image is courtesy of an anonymous Abreview.

    See Abreview

References for Anti-ADAM17 antibody (ab2051)

This product has been referenced in:
  • Cai Z  et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration. Cell Res N/A:N/A (2016). Read more (PubMed: 27444869) »
  • Corbett GT  et al. Activation of peroxisome proliferator-activated receptor a stimulates ADAM10-mediated proteolysis of APP. Proc Natl Acad Sci U S A 112:8445-50 (2015). WB ; Mouse . Read more (PubMed: 26080426) »

See all 21 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Treatment
LPS 5mg/kg IV for liver
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Apr 24 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization
No
Specification
Intestine
Fixative
Formaldehyde
Username

Dr. Pilar Alzuguren

Verified customer

Submitted Aug 10 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% in TGS)
Sample
Mouse Tissue lysate - other (Whole Hippocampus - Cytosolic and Membrane Fractio)
Specification
Whole Hippocampus - Cytosolic and Membrane Fractio
Blocking step
Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Grant Corbett

Verified customer

Submitted Jul 17 2014

Application
Western blot
Loading amount
200 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Specification
hepatocytes
Treatment
100 U/ml IL-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 07 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary astrocytes)
Loading amount
2 µg
Specification
Primary astrocytes
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primary astrocytes)
Specification
primary astrocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - TX100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Various cell lines)
Loading amount
50 µg
Specification
Various cell lines
Gel Running Conditions
Reduced Denaturing (5-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Mar 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (breast)
Loading amount
20 µg
Specification
breast
Gel Running Conditions
Non-reduced Denaturing (4-12% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human keratinocytes (HaCaT))
Loading amount
20000 cells
Specification
Human keratinocytes (HaCaT)
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 5
Username

Abcam user community

Verified customer

Submitted Aug 14 2006

Thank you for your enquiry. The anti-TACE antibody has been tested in mouse lung, brain, liver, kidney, spleen, and 3T3 cells.

1-10 of 16 Abreviews or Q&A

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