Overview

  • Product name
  • Description
    Rabbit polyclonal to ADAM17
  • Specificity
    This antibody recognizes ADAM17, but does not react with other ADAMs.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Rat, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human ADAM17. Only the full length ADAM17 contains the cytoplasmic epitope for this antibody.
    (Peptide available as ab41214)

Properties

Applications

Our Abpromise guarantee covers the use of ab39162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 93 kDa). 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
  • Tissue specificity
    Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
  • Sequence similarities
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • Domain
    Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
    Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
    • A disintegrin and metalloproteinase domain 17 antibody
    • ADA17_HUMAN antibody
    • ADAM 17 antibody
    • ADAM metallopeptidase domain 17 antibody
    • ADAM17 antibody
    • ADAM17 protein antibody
    • CD 156b antibody
    • CD156b antibody
    • CD156b antigen antibody
    • CSVP antibody
    • Disintegrin and metalloproteinase domain-containing protein 17 antibody
    • MGC71942 antibody
    • NISBD antibody
    • NISBD1 antibody
    • Snake venom like protease antibody
    • Snake venom-like protease antibody
    • TACE antibody
    • TNF alpha convertase antibody
    • TNF alpha converting enzyme antibody
    • TNF-alpha convertase antibody
    • TNF-alpha-converting enzyme antibody
    • Tumor Necrosis Factor Alpha Converting Enzyme antibody
    see all

Images

  • ab39162 was used to detect mouse ADAM17 transiently overexpressed in Hela cells. Ab39162 was incubated with the cells at 10 µg/ml for 1 hour at 22°C. For further details please refer to the Abreview.

  • Ab39162 staining human normal colon. Staining is localised to membrane.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

This product has been referenced in:
  • Hurst LA  et al. TNFa drives pulmonary arterial hypertension by suppressing the BMP type-II receptor and altering NOTCH signalling. Nat Commun 8:14079 (2017). WB . Read more (PubMed: 28084316) »
  • Shirakabe K  et al. Mechanistic insights into ectodomain shedding: susceptibility of CADM1 adhesion molecule is determined by alternative splicing and O-glycosylation. Sci Rep 7:46174 (2017). Read more (PubMed: 28393893) »

See all 12 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
Loading amount
100000 cells
Specification
rat vascular smooth muscle cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Verified customer

Submitted Feb 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PANC-1 (Human pancreatic carcinoma, epithelial-lik)
Specification
PANC-1 (Human pancreatic carcinoma, epithelial-lik
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Submitted Jan 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080+MCF7)
Loading amount
10 µg
Specification
HT1080+MCF7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Oct 26 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y cells)
Loading amount
50 µg
Specification
SH-SY5Y cells
Gel Running Conditions
Reduced Denaturing (10-20% tris-tricine)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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Submitted May 12 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted May 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HT1080 Cell line)
Total protein in input
100000 cells
Specification
HT1080 Cell line
Treatment
Pulse labelled with 35S-Met for one hour
Immuno-precipitation step
Protein G
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Submitted Jan 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela Cells transiently overexpressing mouse ADAM17)
Specification
Hela Cells transiently overexpressing mouse ADAM17
Fixative
Methanol
Permeabilization
No
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Submitted Mar 05 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB231 Cell Line)
Loading amount
30000 cells
Specification
MDA-MB231 Cell Line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Sep 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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