For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide based on the activation site (cysteine switch and furin cleavage site) of human ADAM17.
(Peptide available as ab41215.)
Our Abpromise guarantee covers the use of ab39163 in the following tested applications.
|WB||1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 93 kDa). 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 120 kDa in reduced Western blots of conditioned media or cell lysates, which is converted to a 55-60 kDa band.Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
The image shows ab39163 detecting mouse ADAM17 expressed in transfected HeLa cells. The cells were fixed in methanol and incubated with 10 µg/ml ab39163 for 1 hour at 20°C.
Image taken from an anonymous Abreview submitted in September 2007