• Product nameAnti-ADAM17 antibody
    See all ADAM17 primary antibodies
  • Description
    Rabbit polyclonal to ADAM17
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to C-terminal residues of human ADAM17

  • Positive control
    • Skeletal muscle



Our Abpromise guarantee covers the use of ab78162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at a concentration of 1 µg/ml.
    IHC-P: Use at a concentration of 20 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
    • Tissue specificityUbiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
    • Sequence similaritiesContains 1 disintegrin domain.
      Contains 1 peptidase M12B domain.
    • DomainMust be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      The precursor is cleaved by a furin endopeptidase.
      Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
      • A disintegrin and metalloproteinase domain 17 antibody
      • ADA17_HUMAN antibody
      • ADAM 17 antibody
      • ADAM metallopeptidase domain 17 antibody
      • ADAM17 antibody
      • ADAM17 protein antibody
      • CD 156b antibody
      • CD156b antibody
      • CD156b antigen antibody
      • CSVP antibody
      • Disintegrin and metalloproteinase domain-containing protein 17 antibody
      • MGC71942 antibody
      • NISBD antibody
      • NISBD1 antibody
      • Snake venom like protease antibody
      • Snake venom-like protease antibody
      • TACE antibody
      • TNF alpha convertase antibody
      • TNF alpha converting enzyme antibody
      • TNF-alpha convertase antibody
      • TNF-alpha-converting enzyme antibody
      • Tumor Necrosis Factor Alpha Converting Enzyme antibody
      see all

    Anti-ADAM17 antibody images

    • ab78162, used in immunohistochemistry at 20 µg/ml on formalin-fixed, paraffin embedded skeletal muscle

    References for Anti-ADAM17 antibody (ab78162)

    ab78162 has not yet been referenced specifically in any publications.

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    Thank you for your enquiry. The antigen retrieval method used is as below: 1) Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes (vegetable steamer) 2) Remove from heat and let stand at room temperature in buffer - 20...

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