| | | | Immunocytochemistry/ Immunofluorescence - ADAM17 antibody - Cytoplasmic domain (ab39162)
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| Product Name | ADAM17 antibody - Cytoplasmic domain |
| | See all ADAM17 antibodies (12)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to ADAM17 - Cytoplasmic domain |
| Immunogen | Synthetic peptide based on the cytoplasmic domain of full length human ADAM17.
(Peptide available as ab41214.) |
| Reacts with
(species key) | Hu, Rat, Pig |
| Specificity | This antibody recognizes ADAM17, but does not react with other ADAMs. We have a range of domain specific antibodies for this target. For a full list please see all ADAM17 antibodies |
| Epitope | Only the full length ADAM17 contains the cytoplasmic epitope for this antibody. |
| Tested applications
(see key) | ICC/IF, IP, WB |
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| Abreviews | |
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| Application notes
(see key) | Recommended dilutions
ICC/IF: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
WB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | Membrane; single pass type I membrane protein. |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Signal transduction >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Stem Cells >> Signaling Pathways >> Notch >> Secreted
Signal Transduction >> Adapters >> Transmembrane
Neuroscience >> Neurodegeneration >> Alzheimer's >> Other |
| Relevance | ADAM17, also known as TNF alpha Convertase, or TACE, was first cloned from human epithelial cells. The ADAMs proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane and Cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". ADAM17 contains a cleavage site for prohormone convertases (furin), and the majority of ADAM17 is secreted as activated enzyme, cleaved at the furin site. ADAM17 is known to associate with MAD2 via the Cytoplasmic domain, implying cellular regulation pathways. ADAM17 is expressed in a wide range of cells in culture. Three human ADAM17 sequences encode proteins with different cytoplasmic domains, as is the case for other ADAMs proteases. The 824 amino acid sequence encodes a 92,960 Dalton protein, the 807 AA sequence 91,003 Dalton, and the 694 AA sequence 78,543 Dalton predicted MW. Glycosylation and other post translational modifications increase the apparent molecular weight on PAGE gels. Mouse and rat ADAM17 sequences are both 827 AA, predicting 93,073 and 93,017 Dalton proteins, respectively. Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis. |
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAM17 antibody - Cytoplasmic domain is on this datasheet. But please do contact us if you would like any reassurance! |
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| See below for ADAM17 antibody - Cytoplasmic domain images, references, products related to ab39162 and other tools. |
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ADAM17 antibody - Cytoplasmic domain images: |
 Immunocytochemistry/ Immunofluorescence - ADAM17 antibody - Cytoplasmic domain (ab39162)
ab39162 was used to detect mouse ADAM17 transiently overexpressed in Hela cells. Ab39162 was incubated with the cells at 10 µg/ml for 1 hour at 22°C. For further details please refer to the Abreview. Image taken from Abreview submitted in February 2008 |
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| Search PubMed (MEDLINE) for references to ADAM17 |
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