Anti-ADAM20 antibody (ab197829)
Key features and details
- Rabbit polyclonal to ADAM20
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADAM20 antibody -
Description
Rabbit polyclonal to ADAM20 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein corresponding to Human ADAM20 (internal sequence). Gene Accssion: BC025378.
Database link: O43506 -
Positive control
- PC3 and HeLa cell lysates; Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/25 - 1/100.
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WB |
1/100 - 1/500. Predicted molecular weight: 82 kDa.
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Notes |
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IHC-P
1/25 - 1/100. |
WB
1/100 - 1/500. Predicted molecular weight: 82 kDa. |
Target
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Function
May be involved in sperm maturation and/or fertilization. -
Tissue specificity
Testis specific. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
Domain
A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsHas no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8748 Human
- Omim: 603712 Human
- SwissProt: O43506 Human
- Unigene: 177984 Human
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Alternative names
- A disintegrin and metalloproteinase domain 20 antibody
- A disintegrin and metalloproteinase domain 20 preproprotein antibody
- ADA20_HUMAN antibody
see all
Images
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All lanes : Anti-ADAM20 antibody (ab197829) at 1/100 dilution
Lane 1 : PC3 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 82 kDa
Exposure time: 10 secondsGel: 8% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADAM20 with ab197829 at 1/30.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab197829 has not yet been referenced specifically in any publications.