Anti-ADAM20 antibody - Catalytic domain (ab28295)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab28295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: A recommended starting concentration for Western Blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 82 kDa. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. This product recognizes bands of 95 kDa, 84 kDa (major band), and breakdown products at 50 kDa, 34 kDa in reduced Western Blots of cell lysates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay be involved in sperm maturation and/or fertilization.
    • Tissue specificityTestis specific.
    • Sequence similaritiesContains 1 disintegrin domain.
      Contains 1 EGF-like domain.
      Contains 1 peptidase M12B domain.
    • DomainA tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
      The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • A disintegrin and metalloproteinase domain 20 antibody
      • A disintegrin and metalloproteinase domain 20 preproprotein antibody
      • ADA20_HUMAN antibody
      • ADAM 20 antibody
      • ADAM metallopeptidase domain 20 antibody
      • ADAM20 antibody
      • Disintegrin and metalloproteinase domain-containing protein 20 antibody
      • EC 3.4.24. antibody
      • OTTHUMP00000236151 antibody
      see all

    Anti-ADAM20 antibody - Catalytic domain images

    • All lanes : Anti-ADAM20 antibody - Catalytic domain (ab28295) at 1/1000 dilution

      Lane 1 : Mouse Tetis Lysate
      Lane 2 : Mouse Epididymis Lysate

      Lysates/proteins at 20 µl per lane.


      Predicted band size : 82 kDa

    References for Anti-ADAM20 antibody - Catalytic domain (ab28295)

    ab28295 has not yet been referenced specifically in any publications.

    Product Wall

    Dear Cecil In response to your query, I'm afraid I can't provide you with more specific details on the peptide sequence because these amino acid sequences are proprietary. The only information I can provide you with is the following details: ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"