Anti-ADAM22 antibody (ab227546)
Key features and details
- Rabbit polyclonal to ADAM22
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ADAM22 antibody
See all ADAM22 primary antibodies -
Description
Rabbit polyclonal to ADAM22 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human -
Positive control
- WB: Human brain tissue extract, U87-MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. IHC-Fr: Embryonic mouse brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 100 kDa.
Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature. |
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IHC-Fr |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 100 kDa. Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature. |
IHC-Fr
1/100 - 1/1000. |
Target
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Function
Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1. -
Tissue specificity
Highly expressed in the brain and in some high-grade but not low-grade gliomas. Detected slightly or not at all in other tissues. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 53616 Human
- Entrez Gene: 11496 Mouse
- Omim: 603709 Human
- SwissProt: Q9P0K1 Human
- SwissProt: Q9R1V6 Mouse
- Unigene: 592282 Human
- Unigene: 275895 Mouse
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Alternative names
- A disintegrin and metalloproteinase domain 22 antibody
- ADA22_HUMAN antibody
- ADAM 22 antibody
see all
Images
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All lanes : Anti-ADAM22 antibody (ab227546) at 1/500 dilution
Lane 1 : U87-MG whole cell extract
Lane 2 : SK-N-SH whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody at 1/10000 dilution
Predicted band size: 100 kDa10% SDS gel
Running condition: 80V, 15min; 140V, 40 min
Primary antibody incubation: 4C overnight
Washing condition: 5 mL TBST, 4 x 5 minutes -
Anti-ADAM22 antibody (ab227546) at 1/500 dilution + Human brain tissue extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 100 kDa -
Frozen sectioned embryonic mouse brain (mE18.5) stained for ADAM22 with ab227546 (1/500 dilution) (red) in immunohistochemical analysis. Counterstains: DAPI (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab227546 has been referenced in 1 publication.
- Li J et al. miR-449a Suppresses Tamoxifen Resistance in Human Breast Cancer Cells by Targeting ADAM22. Cell Physiol Biochem 50:136-149 (2018). PubMed: 30278449