Anti-ADAM22 antibody (ab231340)
Key features and details
- Rabbit polyclonal to ADAM22
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-ADAM22 antibody
See all ADAM22 primary antibodies -
Description
Rabbit polyclonal to ADAM22 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human ADAM22 aa 350-600. (Expressed in E.coli).
Database link: Q9P0K1 -
Positive control
- IHC-P: Human liver tissue. WB: Pig cerebrum lysate; Recombinant human ADAM22 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231340 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 100 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 100 kDa. |
Target
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Function
Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1. -
Tissue specificity
Highly expressed in the brain and in some high-grade but not low-grade gliomas. Detected slightly or not at all in other tissues. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 53616 Human
- Entrez Gene: 100513676 Pig
- Omim: 603709 Human
- SwissProt: Q9P0K1 Human
- Unigene: 592282 Human
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Alternative names
- A disintegrin and metalloproteinase domain 22 antibody
- ADA22_HUMAN antibody
- ADAM 22 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human liver tissue stained for ADAM22 using ab231340 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-ADAM22 antibody (ab231340) at 2 µg/ml + Pig cerebrum lysate.
Predicted band size: 100 kDa -
Anti-ADAM22 antibody (ab231340) at 2 µg/ml + Recombinant human ADAM22 protein.
Predicted band size: 100 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab231340 has been referenced in 1 publication.
- Fels E et al. Dysregulation of the hippocampal neuronal network by LGI1 auto-antibodies. PLoS One 17:e0272277 (2022). PubMed: 35984846