Overview

  • Product name
  • Description
    Rabbit polyclonal to ADAM22
  • Specificity
    Reacts specifically with human 100 kDa protein. Cross reacts with ADAM 22 protein from mouse, rat and other isoforms.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the C terminal domain of human ADAM22.

Properties

Applications

Our Abpromise guarantee covers the use of ab66497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 100 kDa.
ICC/IF 1/1000.
IHC-P 1/2000.

Target

  • Function
    Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.
  • Tissue specificity
    Highly expressed in the brain and in some high-grade but not low-grade gliomas. Detected slightly or not at all in other tissues.
  • Sequence similarities
    Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase domain 22 antibody
    • ADA22_HUMAN antibody
    • ADAM 22 antibody
    • ADAM metallopeptidase domain 22 antibody
    • adam22 antibody
    • and cysteine-rich protein 2 antibody
    • Disintegrin and metalloproteinase domain-containing protein 22 antibody
    • disintegrin-like antibody
    • MDC 2 antibody
    • MDC2 antibody
    • Metalloproteinase disintegrin ADAM22 3 antibody
    • Metalloproteinase like disintegrin like and cysteine rich protein 2 antibody
    • Metalloproteinase-disintegrin ADAM22-3 antibody
    • Metalloproteinase-like antibody
    • MGC149832 antibody
    see all

Images

  • ICC/IF image of ab66497 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66497, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Ab66497 staining human normal cerebellum tissue. Staining is localized to cellular membrane-bound.
    Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

ab66497 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tooth)
Antigen retrieval step
None
Permeabilization
No
Specification
Tooth
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Dec 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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