Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab28304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesICC/IF: Use at a concentration of 1 µg/ml.
    WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Predicted molecular weight: 92 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceADAM23 is a non-catalytic metalloprotease-like protein. It is highly expressed in the brain, primarily in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex and occipital pole, and weakly expressed in the heart. 3 isoforms are produced by alternative splicing, alpha, beta and gamma. ADAM23 may play a role in cell-cell and cell-matrix interactions.
    • Cellular localizationSingle pass type I membrane protein. 3 isoforms produced by alternative splicing, gamma isoform is secreted.
    • Database links
    • Alternative names
      • A disintegrin and metalloproteinase domain 23 antibody
      • ADAM metallopeptidase domain 23 antibody
      • Disintegrin and metalloproteinase domain containing protein 23 antibody
      • MDC 3 antibody
      • MDC3 antibody
      • Metalloproteinase like disintegrin like and cysteine rich protein 3 antibody
      see all

    Anti-ADAM23 antibody images

    • ICC/IF image of ab28304 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28304, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-ADAM23 antibody (ab28304)

    ab28304 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"