Anti-ADAM23 antibody - Cytoplasmic domain (ab28302)

Overview

  • Product nameAnti-ADAM23 antibody - Cytoplasmic domain
    See all ADAM23 primary antibodies
  • Description
    Rabbit polyclonal to ADAM23 - Cytoplasmic domain
  • Specificityab28302 recognises the Propeptide domain of ADAM23.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to the cytoplasmic domain of human ADAM23.

Properties

Applications

Our Abpromise guarantee covers the use of ab28302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 75 kDa (predicted molecular weight: 92 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • RelevanceADAM23 is a non-catalytic metalloprotease-like protein. It is highly expressed in the brain, primarily in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex and occipital pole, and weakly expressed in the heart. 3 isoforms are produced by alternative splicing, alpha, beta and gamma. ADAM23 may play a role in cell-cell and cell-matrix interactions.
  • Cellular localizationSingle pass type I membrane protein. 3 isoforms produced by alternative splicing, gamma isoform is secreted.
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase domain 23 antibody
    • ADAM metallopeptidase domain 23 antibody
    • Disintegrin and metalloproteinase domain containing protein 23 antibody
    • MDC 3 antibody
    • MDC3 antibody
    • Metalloproteinase like disintegrin like and cysteine rich protein 3 antibody
    see all

Anti-ADAM23 antibody - Cytoplasmic domain images

  • ICC/IF image of ab28302 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28302, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ADAM23 antibody - Cytoplasmic domain (ab28302) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Rat Cortex Tissue Lysate
    Lane 3 : Human brain amygdala tissue lysate (alzheimer) - membrane extract (ab30118)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 92 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa,68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-ADAM23 antibody - Cytoplasmic domain (ab28302)

ab28302 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Kidney)
Permeabilization Yes - 0.1% troton x-100
Specification Kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 06 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 24 2016

Application Western blot
Sample Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 09 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 04 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"