Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Overview
Product name
Anti-ADAM28 antibody - Aminoterminal end
See all ADAM28 products (3) ...
Description
Rabbit polyclonal to ADAM28 - Aminoterminal end
Specificity
ab28292 recognises the Aminoterminal region of ADAM28.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to the Propeptide domain of human ADAM28.
(Peptide available as ab41223.)
Positive control
Western blot: RD (rhabdomyosarcoma, human, embryonal, epithelial) cell lysates and A-431 (epidermoid carcinoma, human) cell lysates
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
-
Western blot - ADAM28 antibody - Aminoterminal end (ab28292)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Predicted molecular weight: 87 kDa.(1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Note: for the full length ADAM28: glycosylation and cyteine-rich regions make the reduced protein run at 114 kDa unprocessed and 84 kDa processed forms. For the soluble ADAM28, under reducing conditions it is seen as a 92 kDa zymogen and 68 kDa furin processed form. Dilution optimised using Chromogenic detection.)
ICC/IF: Use at an assay dependent concentration.
Target
Relevance
ADAM28, also known as eMDCII, MDC-Ls and TECADAM, was first described in lymphocytes, also in Thymic Endothelial Cells (TECADAM), as well as in the epididymus. Later works describe two forms of ADAM28: ADAM28-S and ADAM28-L (short and long forms). The short form is a soluble, alternatively spliced form lacking the transmembrane and cytoplasmic domains. Message levels of the short form were found to be much lower than the long form in the tissues studied, but these may be tissue-specific differences. In humans, ADAM28 is reported in lymphocytes by some authors and to be absent from mature lymphocytes by others. The thyroid, lung, trachea, esophagus, and stomach have been shown to express human ADAM28 message. Knockout mice lacking B cells and T cells express ADAM28 message in the thyroid, lung, trachea, esophagus, and stomach. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs) the functions of ADAM28 are still poorly understood. ADAM28 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Other ADAMs family members (ADAM10, ADAM17) have been more thoroughly studied and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM28 (ADAM28 Long) sequence codes for a 775 amino acid protein with a predicted mass is 87.2 kDa, and a pI of 6.50. The soluble ADAM28 (ADAM-28 Short) is a 540 amino acid sequence, with a predicted mass of 61.3 kDa, and a pI of 6.58. Other forms of ADAM28 have been reported with slightly different carboxyterminal regions. ADAM28 may play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. It may be involved in sperm maturation. There are 2 isoforms of ADAM28: one short secreted and one long membrane bound.
Cellular localization
Cell Membrane and Secreted
Alternative names
- A disintegrin and metalloproteinase domain 28 antibody
- ADAM23 antibody
- MDC L antibody
- Metalloproteinase like disintegrin like and cysteine rich protein L antibody
Database links
- Entrez Gene: 10863 Human
- Omim: 606188 Human
- SwissProt: Q9UKQ2 Human
Anti-ADAM28 antibody - Aminoterminal end images:
Western blot - ADAM28 antibody - Aminoterminal end (ab28292)
All lanes : Anti-ADAM28 antibody - Aminoterminal end (ab28292) at 1 µg/ml
Lane 1 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)
Lane 2 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 87 kDa
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 12 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
The band observed at 66 kDa could potentially be a cleaved form of ADAM28 due to the presence of an 18 amino acid signal peptide and a 180 amino acid propeptide.
Protocols
References for Anti-ADAM28 antibody - Aminoterminal end (ab28292)
This product has been referenced in:
- Yang MHet al. Characterization of ADAM28 as a biomarker of bladder transitional cell carcinomas by urinary proteome analysis. Biochem Biophys Res Commun 411:714-20 (2011). WB; Human.Read more (PubMed: 21782798) »
See 1 publication for this product
Publishing research using ab28292? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"