Overview

  • Product nameAnti-ADAM28 antibody - Aminoterminal end
    See all ADAM28 primary antibodies
  • Description
    Rabbit polyclonal to ADAM28 - Aminoterminal end
  • Specificityab28292 recognises the Aminoterminal region of ADAM28.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to the Propeptide domain of human ADAM28.

    (Peptide available as ab41223.)

  • Positive control
    • Western blot: RD (rhabdomyosarcoma, human, embryonal, epithelial) cell lysates and A-431 (epidermoid carcinoma, human) cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab28292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 87 kDa. 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Note: for the full length ADAM28: glycosylation and cyteine-rich regions make the reduced protein run at 114 kDa unprocessed and 84 kDa processed forms. For the soluble ADAM28, under reducing conditions it is seen as a 92 kDa zymogen and 68 kDa furin processed form. Dilution optimised using Chromogenic detection.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceADAM28, also known as eMDCII, MDC-Ls and TECADAM, was first described in lymphocytes, also in Thymic Endothelial Cells (TECADAM), as well as in the epididymus. Later works describe two forms of ADAM28: ADAM28-S and ADAM28-L (short and long forms). The short form is a soluble, alternatively spliced form lacking the transmembrane and cytoplasmic domains. Message levels of the short form were found to be much lower than the long form in the tissues studied, but these may be tissue-specific differences. In humans, ADAM28 is reported in lymphocytes by some authors and to be absent from mature lymphocytes by others. The thyroid, lung, trachea, esophagus, and stomach have been shown to express human ADAM28 message. Knockout mice lacking B cells and T cells express ADAM28 message in the thyroid, lung, trachea, esophagus, and stomach. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs) the functions of ADAM28 are still poorly understood. ADAM28 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Other ADAMs family members (ADAM10, ADAM17) have been more thoroughly studied and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM28 (ADAM28 Long) sequence codes for a 775 amino acid protein with a predicted mass is 87.2 kDa, and a pI of 6.50. The soluble ADAM28 (ADAM-28 Short) is a 540 amino acid sequence, with a predicted mass of 61.3 kDa, and a pI of 6.58. Other forms of ADAM28 have been reported with slightly different carboxyterminal regions. ADAM28 may play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. It may be involved in sperm maturation. There are 2 isoforms of ADAM28: one short secreted and one long membrane bound.
  • Cellular localizationCell Membrane and Secreted
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase domain 28 antibody
    • ADAM 28 antibody
    • ADAM metallopeptidase domain 28 antibody
    • ADAM23 antibody
    • Disintegrin and metalloproteinase domain containing protein 28 antibody
    • eMDC II antibody
    • eMDCII antibody
    • epididymal Metalloproteinase like disintegrin like and cysteine rich protein II antibody
    • MDC L antibody
    • MDCL antibody
    • Metalloproteinase like disintegrin like and cysteine rich protein L antibody
    see all

Anti-ADAM28 antibody - Aminoterminal end images

  • All lanes : Anti-ADAM28 antibody - Aminoterminal end (ab28292) at 1 µg/ml

    Lane 1 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 87 kDa
    Observed band size : 66 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 12 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • Anti-ADAM28 antibody - Aminoterminal end (ab28292) at 1/1000 dilution + whole cell lysate prepared from EL4 cells at 100000 cells

    Secondary
    HRP conjugated donkey anti-rabbit polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 87 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-ADAM28 antibody - Aminoterminal end (ab28292)

This product has been referenced in:
  • Yang MH  et al. Characterization of ADAM28 as a biomarker of bladder transitional cell carcinomas by urinary proteome analysis. Biochem Biophys Res Commun 411:714-20 (2011). WB ; Human . Read more (PubMed: 21782798) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (EL4)
Loading amount 100000 cells
Specification EL4
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted May 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela Cells transiently overexpressing human ADAM28)
Specification Hela Cells transiently overexpressing human ADAM28
Fixative Methanol
Permeabilization No
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Submitted Aug 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"