ADAM29 antibody - Cytoplasmic domain (ab28290)

Product Name 

ADAM29 antibody - Cytoplasmic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAM29 - Cytoplasmic domain

Immunogen 

Synthetic peptide corresponding to the cytoplasmic domain of human ADAM29.

(Peptide available as ab41224.)

Reacts with 
(species key)

Hu

Specificity 

ab28290 recognises the Cytoplasmic domain of ADAM29. It will not recognize the shed form of ADAM29.

We have a range of domain specific antibodies for this target. For a full list please see all ADAM29 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 93 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Type I membrane protein

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family

Relevance 

ADAM29 was first described almost simultaneously by two different groups that analyzed EST libraries for homology to other ADAMS proteins. Both groups identified several splice variants, the variations all being in the length of the cytoplasmic domain, and both found the messages limited to testis. Later, ADAM29 message was shown to be elevated in chronic lympocytic leukemia (CLL), and is thought to be a useful marker for CLL. The ADAM29 is is thus not specifically limited to testis, but to date there are no publications on ADAM-29 protein expression or localization. A member of the metalloproteinase family containing disintegrin-like domains (ADAMS), the function of ADAM29 is still poorly understood. ADAM29 does not contain the canonical HExxHxxxxxH zinc metalloproteinase motif; rather ADAM29 contains a HHxxHHxxxxxH sequence, and as a result is thought to be proteolytically inactive. Other ADAMs family members (ADAM10, ADAM17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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