The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 88 kDa.
Experiments gave bands at approx 80-90kDa and strong ladder from 26 to 38kDa in Human Prostate lysates after 0.3ug/ml antibody staining. The observation of multiple bands and the 38 kDa band correspond to earlier findings in literature (Powell et al, Am J Respir Cell Mol Biol. 2004 Jul;31(1):13-21; PMID: 14742294).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.
Involvement in disease
Genetic variations in ADAM33 are associated with susceptibility to asthma (ASTHMA) [MIM:600807]. The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
The precursor is cleaved by a furin endopeptidase.
A disintegrin and metalloproteinase domain 33 antibody
ADAM 33 antibody
ADAM metallopeptidase domain 33 antibody
Disintegrin and metalloproteinase domain-containing protein 33 antibody
Disintegrin and reprolysin metalloproteinase family protein antibody
Metalloprotease disintegrin antibody
Western blot - Anti-ADAM33 antibody (ab36172)
All lanes : Anti-ADAM33 antibody (ab36172) at 0.3 µg/ml
Lane 1 : Human Prostate lysate (35µg protein in RIPA buffer) with no blocking from immunizing peptide Lane 2 : Human Prostate lysate (35µg protein in RIPA buffer) with blocking from immunizimg peptide
Predicted band size: 88 kDa
Primary incubation was 1 hour. Detected by chemiluminescence. Bands at approx 80-90kDa and strong ladder from 26 to 38kDa in Human Prostate lysates after 0.3µg/ml antibody staining. The observation of multiple bands and the 38 kDa band correspond to earlier findings in literature (Powell et al, Am J Respir Cell Mol Biol. 2004 Jul;31(1):13-21; PMID: 14742294).