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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Overview
Product name
Anti-ADAM33 antibody - Aminoterminal end
See all ADAM33 products (4) ...
Description
Rabbit polyclonal to ADAM33 - Aminoterminal end
Specificity
Recognizes metalloproteinase ADAM33.
Tested applications
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Synthetic peptide from the N terminal region of active Human ADAM33.
(Peptide available as ab41228.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
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Immunocytochemistry/ Immunofluorescence-ADAM33 antibody - Aminoterminal end(ab39191)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab39191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 - 1/5000. Detects a band of approximately 99 kDa (minor band), due to glycosylation and cysteine rich regions, 58 kDa (major band), under reducing conditions. (Predicted molecular weight: 88 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Tissue specificity
Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.
Involvement in disease
Genetic variations in ADAM33 are associated with susceptibility to asthma (ASTHMA) [MIM:600807]. The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ9BZ11
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase domain 33 antibody
- A disintegrin and metalloproteinase domain 33 antibody
- ADA33_HUMAN antibody
see all
Database links
- Entrez Gene: 80332 Human
- Omim: 607114 Human
- SwissProt: A0A1K6 Human
- SwissProt: Q08AM2 Human
- SwissProt: Q6P5U4 Human
- SwissProt: Q9BZ11 Human
- Unigene: 173716 Human
Anti-ADAM33 antibody - Aminoterminal end images:
Immunocytochemistry/ Immunofluorescence-ADAM33 antibody - Aminoterminal end(ab39191)
ICC/IF image of ab39191 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39191, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-ADAM33 antibody - Aminoterminal end (ab39191)
ab39191 has not yet been referenced specifically in any publications.
Publishing research using ab39191? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"