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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Overview
Product name
Anti-ADAM33 antibody - Cytoplasmic domain
See all ADAM33 products (4) ...
Description
Rabbit polyclonal to ADAM33 - Cytoplasmic domain
Tested applications
WB, IHC-R, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from Human ADAM33 based on the cytoplasmic domain.
(Peptide available as ab41229.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ADAM33 antibody - Cytoplasmic domain(ab39193)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab39193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IHC-R
ICC/IF
Application notes
ICC/IF: Use at an assay dependent dilution.
IHC-R: Use at an assay dependent dilution.
IHC-P: Use at 0.5ug/ml (see ref Susan C. Foley MD et al)
WB: 1/1000 - 1/5000. Detects a band of approximately 99 kDa (minor form), due to glycosylation and cysteine rich regions, and a band at 58 kDa (major band), under reducing conditions. (Predicted molecular weight: 88 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Tissue specificity
Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.
Involvement in disease
Genetic variations in ADAM33 are associated with susceptibility to asthma (ASTHMA) [MIM:600807]. The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ9BZ11
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase domain 33 antibody
- A disintegrin and metalloproteinase domain 33 antibody
- ADA33_HUMAN antibody
see all
Database links
- Entrez Gene: 80332 Human
- Omim: 607114 Human
- SwissProt: A0A1K6 Human
- SwissProt: Q08AM2 Human
- SwissProt: Q6P5U4 Human
- SwissProt: Q9BZ11 Human
- Unigene: 173716 Human
Anti-ADAM33 antibody - Cytoplasmic domain images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ADAM33 antibody - Cytoplasmic domain(ab39193)
Ab39193 staining human normal myometrium. Staining is localized to the membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
References for Anti-ADAM33 antibody - Cytoplasmic domain (ab39193)
This product has been referenced in:
- Foley SCet al. Increased expression of ADAM33 and ADAM8 with disease progression in asthma. J Allergy Clin Immunol 119:863-71 (2007). IHC-P; Human.Read more (PubMed: 17339047) »
See 1 publication for this product
Publishing research using ab39193? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"