Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 89 kDa).Can be blocked with Human ADAM8 peptide (ab22912). Approx 100kDa band observed in Human Bone Marrow and Peripheral Blood Mononucleocytes lysates

Target

  • FunctionPossible involvement in extravasation of leukocytes.
  • Tissue specificityExpressed on neutrophils and monocytes.
  • Sequence similaritiesContains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A Disintegrin And Metalloproteinase domain 8 antibody
    • ADAM 8 antibody
    • ADAM 8 precursor antibody
    • ADAM metallopeptidase domain 8 antibody
    • Adam8 antibody
    • ADAM8 protein antibody
    • ADAM8_HUMAN antibody
    • CD 156 antibody
    • CD156 antibody
    • CD156a antibody
    • CD156a antigen antibody
    • Cell surface antigen MS2 antibody
    • Disintegrin and metalloproteinase domain-containing protein 8 antibody
    • Human leukocyte differentiation antigen antibody
    • Macrophage cysteine rich glycoprotein antibody
    • MGC134985 antibody
    • MS 2 antibody
    • MS2 antibody
    see all

Anti-ADAM8 antibody images



  • Predicted band size : 89 kDa

References for Anti-ADAM8 antibody (ab2475)

ab2475 has not yet been referenced specifically in any publications.

Product Wall

Thanks for your email and for your patience. Your positive control looks very similar to the image on the datasheet. I recall that previously you were not able to detect any bands at all with ab2475 and the human brain lysate, thus why I sent you the ...

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Thank you very much for your email and for your patience. We value your feedback and take your comments very seriously. A couple of things that I noticed from your results - I do see a band at 90 kDa in your positive control, but there is no band at 90...

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Thank you for your message. After reviewing your results with the different Adam8 antibodies that you have tried, we're not sure that the R&D antibody results are reliable as the 88kDa band is very weak compared to the other bands. You also mentioned ...

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Thank you very much for the information that you have provided. I am concerned that you got absolutely no bands in the positive control. Your protocol seems fine and you should have expected to see a band at aprroximately 90 kDa with the brain lysate. ...

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Thank you for sending me the details of your protocol. To try to get rid of the non-specific bands that you are seeing, I would like to suggest the following. Decrease the amount of primary that you are using (try 1:1000) and decrease the concentration...

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