• Product nameAnti-ADAMTS1 antibody
    See all ADAMTS1 primary antibodies
  • Description
    Rabbit polyclonal to ADAMTS1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human ADAMTS1.

  • Positive control
    • This antibody gave a positive signal in U20S and A549 whole cell lysates. This antibody gave a positive signal in the following formaldehyde fixed cells: HeLa.



Our Abpromise guarantee covers the use of ab113847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionCleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
  • Sequence similaritiesContains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 3 TSP type-1 domains.
  • DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase with thrombospondin motifs 1 antibody
    • ADAM-TS 1 antibody
    • ADAM-TS1 antibody
    • ADAMTS 1 antibody
    • ADAMTS antibody
    • ADAMTS-1 antibody
    • Adamts1 antibody
    • ATS1_HUMAN antibody
    • C3 C5 antibody
    • KIAA1346, antibody
    • METH 1 antibody
    • METH-1 antibody
    see all

Anti-ADAMTS1 antibody images

  • All lanes : Anti-ADAMTS1 antibody (ab113847) at 1 µg/ml

    Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 105 kDa
    Observed band size : 105 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • ICC/IF image of ab113847 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113847 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ADAMTS1 antibody (ab113847)

ab113847 has not yet been referenced specifically in any publications.

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