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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-ADAMTS1 antibody - Propeptide domain
See all ADAMTS1 products (5) ...
Description
Rabbit polyclonal to ADAMTS1 - Propeptide domain
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
This antibody is made to a synthetic peptide based on the Propeptide domain of human ADAMTS1.
Epitope
This antibody recognizes the first 30 amino acids of the protein.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Use at an assay dependent dilution. Predicted molecular weight: 105 kDa. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Ab39195 recognizes the zymogen of ADAMTS1 at 110-120 kD in reduced Western blots, but not the furin cleaved ADAMTS1. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains.
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ9UHI8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 1 antibody
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 1 antibody
- A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1 antibody
see all
Database links
- Entrez Gene: 9510 Human
- Omim: 605174 Human
- SwissProt: Q9UHI8 Human
- Unigene: 643357 Human
- Unigene: 8230 Human
Protocols
References for Anti-ADAMTS1 antibody - Propeptide domain (ab39195)
ab39195 has not yet been referenced specifically in any publications.
Publishing research using ab39195? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"