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Read our guarantee »Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Anti-ADAMTS1 antibody - Propeptide domain
See all ADAMTS1 products (5) ...
Rabbit polyclonal to ADAMTS1 - Propeptide domain
Reacts with
Human
This antibody is made to a synthetic peptide based on the Propeptide domain of human ADAMTS1.
This antibody recognizes the first 30 amino acids of the protein.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Our Abpromise guarantee covers the use of ab39195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 105 kDa. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Ab39195 recognizes the zymogen of ADAMTS1 at 110-120 kD in reduced Western blots, but not the furin cleaved ADAMTS1. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains.
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
The precursor is cleaved by a furin endopeptidase.
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ9UHI8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
ab39195 has not yet been referenced specifically in any publications.
Publishing research using ab39195? Please let us know so that we can cite the reference in this datasheet
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