ADAMTS1 antibody - Propeptide domain (ab39195)

Product Name 

ADAMTS1 antibody - Propeptide domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAMTS1 - Propeptide domain

Immunogen 

This antibody is made to a synthetic peptide based on the Propeptide domain of human ADAMTS1.

Reacts with 
(species key)

Hu

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS1 antibodies

Epitope 

This antibody recognizes the first 30 amino acids of the protein.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: Use at an assay dependent dilution. Predicted molecular weight: 105 kDa. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Ab39195 recognizes the zymogen of ADAMTS1 at 110-120 kD in reduced Western blots, but not the furin cleaved ADAMTS1. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted. Associated with the extracellular matrix.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

ADAMTS1 is a metalloproteinase of the ADAM (A Disintegrin And Metalloproteinase) family containing disintegrin-like domains. ADAMTS1, also known as METH1, was first described as a protein elevated in invasive mouse tumors. Initial findings indicated a role for ADAMTS1 in tumor progression, since the protein was preferentially expressed in more invasive tumor cell lines. ADAMTS1 is necessary for normal growth, fertility, and organ morphology and function.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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