Overview

  • Product nameAnti-ADAMTS15 antibody
    See all ADAMTS15 primary antibodies
  • Description
    Rabbit polyclonal to ADAMTS15
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Rhesus monkey
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    HSTNRLTLAVAW

    , corresponding to amino acids 592-603 of Human ADAMTS15

  • Positive control
    • 293EBNA cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab28516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 103 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 21791558

Target

  • Tissue specificityExpressed in fetal liver and kidney, but not in any of the adult tissues examined.
  • Sequence similaritiesContains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 3 TSP type-1 domains.
  • DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
    Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase with thrombospondin motifs 15 antibody
    • A disintegrin like and metalloprotease reprolysin type with thrombospondin type 1 motif 15 antibody
    • A disintegrin like and metalloprotease reprolysin type with thrombospondin type 1 motif 15 preproprotein antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 15 antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 15 preproprotein antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 15 antibody
    • ADAM TS 15 antibody
    • ADAM TS15 antibody
    • ADAM-TS 15 antibody
    • ADAM-TS15 antibody
    • ADAMTS 15 antibody
    • ADAMTS-15 antibody
    • ADAMTS15 antibody
    • ATS15_HUMAN antibody
    • MGC126403 antibody
    see all

Anti-ADAMTS15 antibody images

References for Anti-ADAMTS15 antibody (ab28516)

This product has been referenced in:
  • Demircan K  et al. ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury. Biomed Res Int 2014:693746 (2014). WB ; Mouse . Read more (PubMed: 25101296) »
  • Peluffo MC  et al. Systematic analysis of protease gene expression in the rhesus macaque ovulatory follicle: metalloproteinase involvement in follicle rupture. Endocrinology 152:3963-74 (2011). IHC-P ; Rhesus monkey . Read more (PubMed: 21791558) »

See all 3 Publications for this product

Product Wall

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I've heard back from the lab, and the antibody was actually tested on a recombinantly expressed fragment of t...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (MDAMB231 breast cancer cells)
Loading amount 30 µg
Specification MDAMB231 breast cancer cells
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Apr 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"