Overview

  • Product name
  • Description
    Rabbit polyclonal to ADAMTS4
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C-terminal of Human ADAMTS4 (NM_005099).

  • Positive control
    • IHC-P: Human brain tissue. WB: Human fetal brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab84792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P 1/100 - 1/500.

Target

  • Function
    Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
    -Ala-393' site.
  • Tissue specificity
    Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.
  • Sequence similarities
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 1 TSP type-1 domain.
  • Domain
    The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 4 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 4 antibody
    • ADAM TS 4 antibody
    • ADAM TS4 antibody
    • ADAM-TS 4 antibody
    • ADAM-TS4 antibody
    • ADAMTS 2 antibody
    • ADAMTS 4 antibody
    • ADAMTS-4 antibody
    • ADAMTS2 antibody
    • ADAMTS4 antibody
    • ADAMTS4 protein antibody
    • ADMP 1 antibody
    • ADMP-1 antibody
    • ADMP1 antibody
    • Aggrecanase 1 antibody
    • Aggrecanase-1 antibody
    • Aggrecanase1 antibody
    • ATS4_HUMAN antibody
    • KIAA0688 antibody
    see all

Images

  • Anti-ADAMTS4 antibody (ab84792) at 1/1000 dilution + Human fetal brain lysates

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
  • ab84792 at a 1/200 dilution staining ADAMTS4 in Human brain tissue.

References

This product has been referenced in:
  • Wu J  et al. Wnt-ß-catenin signaling pathway inhibition by sclerostin may protect against degradation in healthy but not osteoarthritic cartilage. Mol Med Rep 15:2423-2432 (2017). WB ; Human . Read more (PubMed: 28259981) »
  • Lima MA  et al. Progesterone acts via the progesterone receptor to induce adamts proteases in ovarian cancer cells. J Ovarian Res 9:9 (2016). Read more (PubMed: 26916548) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

We guarantee many of the ADAMTS4 antibodies in our catalog to work in Western Blot on human derived samples. While I admit that I am not an expert on this target I assume that because you are looking to test th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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