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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Overview
Product name
Anti-ADAMTS4 antibody - Amino end of propeptide domain
See all ADAMTS4 products (7) ...
Description
Rabbit polyclonal to ADAMTS4 - Amino end of propeptide domain
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide based on the propeptide domain of human ADAMTS4. (Peptide available as ab41236.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. The full length ADAMTS4 sequence codes for a 837 amino acid protein, with a predicted mass is 90.244 kD, but glycosylation and the abundance of cysteine residues gives ADAMTS4 a greater apparent molecular weight on reduced SDS PAGE gels. This antibody recognizes the zymogen of ADAMTS4 at 98 kD in reduced Western blots, activated forms at 64 kD (major bands), and breakdown products at 30 kD of cell lysates. The antibody detects a band at about 29 kD in cell culture media, which may be the propeptide domain shed after furin cleavage. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
-Ala-393' site.
Tissue specificity
Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 TSP type-1 domain.
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionO75173
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADAMTS 4 antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 antibody
see all
Database links
- Entrez Gene: 9507 Human
- Entrez Gene: 240913 Mouse
- Omim: 603876 Human
- SwissProt: O75173 Human
- SwissProt: Q8NEK2 Human
- SwissProt: Q8BNJ2 Mouse
- Unigene: 211604 Human
- Unigene: 475917 Mouse
Protocols
References for Anti-ADAMTS4 antibody - Amino end of propeptide domain (ab39201)
ab39201 has not yet been referenced specifically in any publications.
Publishing research using ab39201? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"