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Anti-ADAMTS4 antibody - Amino end of propeptide domain
See all ADAMTS4 products (7) ...
Rabbit polyclonal to ADAMTS4 - Amino end of propeptide domain
Reacts with
Human
Predicted to work with
Mouse
Synthetic peptide based on the propeptide domain of human ADAMTS4. (Peptide available as ab41236.)
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Our Abpromise guarantee covers the use of ab39201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. The full length ADAMTS4 sequence codes for a 837 amino acid protein, with a predicted mass is 90.244 kD, but glycosylation and the abundance of cysteine residues gives ADAMTS4 a greater apparent molecular weight on reduced SDS PAGE gels. This antibody recognizes the zymogen of ADAMTS4 at 98 kD in reduced Western blots, activated forms at 64 kD (major bands), and breakdown products at 30 kD of cell lysates. The antibody detects a band at about 29 kD in cell culture media, which may be the propeptide domain shed after furin cleavage. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
-Ala-393' site.
Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 TSP type-1 domain.
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
The precursor is cleaved by a furin endopeptidase.
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionO75173
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
ab39201 has not yet been referenced specifically in any publications.
Publishing research using ab39201? Please let us know so that we can cite the reference in this datasheet
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