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Product Name 

ADAMTS4 antibody - Amino end of propeptide domain

 

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Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAMTS4 - Amino end of propeptide domain

Immunogen 

Synthetic peptide based on the propeptide domain of human ADAMTS4.(Peptide available as ab41236.)

Reacts with 

Hu

Predicted to react 
(species key)

Ms
(Due to sequence homology)

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS4 antibodies

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. The full length ADAMTS4 sequence codes for a 837 amino acid protein, with a predicted mass is 90.244 kD, but glycosylation and the abundance of cysteine residues gives ADAMTS4 a greater apparent molecular weight on reduced SDS PAGE gels. This antibody recognizes the zymogen of ADAMTS4 at 98 kD in reduced Western blots, activated forms at 64 kD (major bands), and breakdown products at 30 kD of cell lysates. The antibody detects a band at about 29 kD in cell culture media, which may be the propeptide domain shed after furin cleavage. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.



Specific protocols
Recommended WB protocol

Cellular localization 

Secreted. Associated with the extracellular matrix.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP

Relevance 

A disintegrin like and metalloproteinase thrombospondin type 1 motif 4 (ADAMTS4 or aggrecanase 1) is a member of the ADAMTS family of multidomain proteins that has demonstrated metalloproteinase functions. The ADAMTS4 protein contains a prohormone convertase cleavage site, thrombospondin 1 like domain, cysteine rich domain, and a propeptide domain. ADAMTS4 activity had been shown to be blocked by TIMP1, 2, 3, and 4.

ADAMTS4 plays an important role in the cleavage of aggrecan, versican and brevican. Studies have determined that aggrecan is cleaved within the interglobular domain of aggrecan at sites Glu(373) Ala(374) and Phe(342) Glu(373). Aggrecan degradation has been linked to the development of rheumatoid arthritis, osteoarthritis, retinal diseases, Alzheimer's and other neurodegenerative diseases.

 

Alternative names for ADAMTS4 antibody - Amino end of propeptide domain (ab39201)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

9507    (Human)
240913    (Mouse)

GeneCard

    

GC01M157937    (Human)

Omim

    

603876    (Human)

SwissProt

    

O75173    (Human)
Q8NEK2    (Human)
Q8BNJ2    (Mouse)

Unigene

    

211604    (Human)
23156    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAMTS4 antibody - Amino end of propeptide domain is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to ADAMTS4

 

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Lysates

 

Immunizing Peptide for ADAMTS4 antibody - Amino end of propeptide domain

ab41236

  

ADAMTS4 peptide (Amino end of propeptide domain)

 

Compatible Secondaries for ADAMTS4 antibody - Amino end of propeptide domain

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other ADAMTS4 Antibodies

See all ADAMTS4 antibodies (3)...

 

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