Anti-ADAMTS4 antibody - Carboxyterminal end (ab28285)

Overview

  • Product nameAnti-ADAMTS4 antibody - Carboxyterminal end
    See all ADAMTS4 primary antibodies
  • Description
    Rabbit polyclonal to ADAMTS4 - Carboxyterminal end
  • Specificityab28285 recognises the Carboxyterminal end of ADAMTS4. This antibody recognises its zymogen and the activated form.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to the carboxyterminal end of Human ADAMTS4. (Peptide available as ab41235.)

Properties

Applications

Our Abpromise guarantee covers the use of ab28285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
IHC-P Use at an assay dependent concentration. PubMed: 21791558

Target

  • FunctionCleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
    -Ala-393' site.
  • Tissue specificityExpressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.
  • Sequence similaritiesContains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 1 TSP type-1 domain.
  • DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 4 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 4 antibody
    • ADAM TS 4 antibody
    • ADAM TS4 antibody
    • ADAM-TS 4 antibody
    • ADAM-TS4 antibody
    • ADAMTS 2 antibody
    • ADAMTS 4 antibody
    • ADAMTS-4 antibody
    • ADAMTS2 antibody
    • ADAMTS4 antibody
    • ADAMTS4 protein antibody
    • ADMP 1 antibody
    • ADMP-1 antibody
    • ADMP1 antibody
    • Aggrecanase 1 antibody
    • Aggrecanase-1 antibody
    • Aggrecanase1 antibody
    • ATS4_HUMAN antibody
    • KIAA0688 antibody
    see all

References for Anti-ADAMTS4 antibody - Carboxyterminal end (ab28285)

This product has been referenced in:
  • Funck-Brentano T  et al. Targeting bone alleviates osteoarthritis in osteopenic mice and modulates cartilage catabolism. PLoS One 7:e33543 (2012). IHC-P ; Mouse . Read more (PubMed: 22432033) »
  • Peluffo MC  et al. Systematic analysis of protease gene expression in the rhesus macaque ovulatory follicle: metalloproteinase involvement in follicle rupture. Endocrinology 152:3963-74 (2011). IHC-P ; Rhesus monkey . Read more (PubMed: 21791558) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Antigen retrieval step None
Sample Mouse Tissue sections (cartilage and meniscus)
Specification cartilage and meniscus
Permeabilization No
Fixative zinc buffered formalin
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Submitted Oct 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"