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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Overview
Product name
Anti-ADAMTS4 antibody - Propeptide domain
See all ADAMTS4 products (7) ...
Description
Rabbit polyclonal to ADAMTS4 - Propeptide domain
Specificity
This antibody is specific for ADAMTS4 (Propeptide domain).
Tested applications
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse
Immunogen
A synthetic peptide based on the propeptide domain of human ADAMTS4. (Peptide available as ab45232.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
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Western blot - ADAMTS4 antibody - Propeptide domain (ab45038)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 90 kDa.(A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Note: Glycosylation and the abundance of cysteine residues gives ADAMTS4 a greater apparent molecular weight on reduced SDS PAGE gels.)
Target
Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
-Ala-393' site.
Tissue specificity
Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 TSP type-1 domain.
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionO75173
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADAMTS 4 antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 antibody
see all
Database links
- Entrez Gene: 9507 Human
- Entrez Gene: 240913 Mouse
- Entrez Gene: 66015 Rat
- Omim: 603876 Human
- SwissProt: O75173 Human
- SwissProt: Q8NEK2 Human
- SwissProt: Q8BNJ2 Mouse
- SwissProt: Q9ESP7 Rat
see all
Anti-ADAMTS4 antibody - Propeptide domain images:
Western blot - ADAMTS4 antibody - Propeptide domain (ab45038)
All lanes : Anti-ADAMTS4 antibody - Propeptide domain (ab45038) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from rat osteosarcoma UMR 106 cells (treated with SF)
Lane 2 : Whole cell lysate prepared from rat osteosarcoma UMR 106 cells (treated with TPA)
Lane 3 : Whole cell lysate prepared from rat osteosarcoma UMR 106 cells (treated with IL1-alpha)
Lane 4 : Whole cell lysate prepared from rat osteosarcoma UMR 106 cells (treated with IL1-beta)
Lane 5 : Whole cell lysate prepared from rat osteosarcoma UMR 106 cells (treated with TNA-alpha)
Lane 6 : Whole cell lysate prepared from rat osteosarcoma UMR 106 cells (treated with RA)
Predicted band size : 90 kDa
Protocols
References for Anti-ADAMTS4 antibody - Propeptide domain (ab45038)
This product has been referenced in:
- Wang Jet al. TNF-a and IL-1ß Promote a Disintegrin-like and Metalloprotease with Thrombospondin Type I Motif-5-mediated Aggrecan Degradation through Syndecan-4 in Intervertebral Disc. J Biol Chem 286:39738-49 (2011).Read more (PubMed: 21949132) »
See 1 publication for this product
Publishing research using ab45038? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"