Overview

  • Product nameAnti-ADAMTS5 antibody
    See all ADAMTS5 primary antibodies
  • Description
    Rabbit polyclonal to ADAMTS5
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human ADAMTS5.

    (Peptide available as ab41036.)

  • Positive control
    • ab41037 gave a positive signal in the following whole cell lysates: Uterus (Human)- normal tissue HeLa (Human epithelial carcinoma cell line) Placenta (Mouse) - normal tissue NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) ab41037 gave a positive signal in the following tissue lysates: Brain (Mouse),Liver (Mouse),Testis (Mouse),Spinal Cord (Mouse),Ovary (Mouse),Brain (Rat),Liver (Rat) ICC/IF: MG-63

Properties

Applications

Our Abpromise guarantee covers the use of ab41037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 73 kDa (predicted molecular weight: 102 kDa).Can be blocked with Human ADAMTS5 peptide (ab41036).

ab41037 appears to detect the 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.

ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionCleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
  • Tissue specificityExpressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.
  • Sequence similaritiesContains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 2 TSP type-1 domains.
  • DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
    • A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase 2) antibody
    • A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 5 antibody
    • ADAM TS 11 antibody
    • ADAM TS 5 antibody
    • ADAM TS5 antibody
    • ADAM-TS 11 antibody
    • ADAM-TS 5 antibody
    • ADAM-TS5 antibody
    • ADAMTS 11 antibody
    • ADAMTS 5 antibody
    • ADAMTS-11 antibody
    • ADAMTS-5 antibody
    • ADAMTS11 antibody
    • Adamts5 antibody
    • ADMP 2 antibody
    • ADMP-2 antibody
    • ADMP2 antibody
    • Aggrecanase 2 antibody
    • Aggrecanase-2 antibody
    • ATS5_HUMAN antibody
    • FLJ36738 antibody
    • Implantin antibody
    • ThromboSpondin motif-5 antibody
    see all

Anti-ADAMTS5 antibody images

  • ICC/IF image of ab41037 stained MG-63 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41037 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab41037 staining ADAMTS5 in mouse knee joint tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and an enzymatic mediated antigen retrieval step was performed using Proteinase K. Samples were then blocked with 5% serum for 10 minutes at room temperature followed by incubation with the primary antibody at 10µg/ml for 1 hour. An undiluted HRP-conjugated human anti-rabbit monoclonal was used as secondary antibody.

    See Abreview

  • All lanes : Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Mouse placenta normal tissue lysate - total protein (ab29139)
    Lane 3 : Uterus (Human) Whole Cell Lysate - normal tissue
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.

    ab41037 appears to detect a 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. The doublet observed in human uterus lysate is possibly due to glycosylation.

  • All lanes : Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 6 : Spinal Cord (Mouse) Tissue Lysate
    Lane 7 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 9 : Brain (Rat) Tissue Lysate - normal tissue
    Lane 10 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)

    ab41037 appears to detect at 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.

  • All lanes : Anti-ADAMTS5 antibody (ab41037) at 1 µg/ml (blocked with 3% Milk)

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

    ab41037 appears to detect at 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.

References for Anti-ADAMTS5 antibody (ab41037)

This product has been referenced in:
  • Yang W  et al. Clock gene Bmal1 modulates human cartilage gene expression by crosstalk with Sirt1. Endocrinology N/A:en20152042 (2016). WB ; Human . Read more (PubMed: 27253997) »
  • Lu W  et al. CXCL12/CXCR4 Axis Regulates Aggrecanase Activation and Cartilage Degradation in a Post-Traumatic Osteoarthritis Rat Model. Int J Mol Sci 17:N/A (2016). Rat . Read more (PubMed: 27690009) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (hippocampus)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification hippocampus
Blocking step licor fluorescence blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Oct 25 2016

Thank you for your call today and for letting us know about the trouble with the new vial of this antibody.

As we discussed, I'm sending a free of charge vial on the order ***, which should arrive tomorrow.

Please keep me updated ab...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (knee joints)
Specification knee joints
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K DAKO S3020
Permeabilization No
Blocking step DEEB DAKO S2003 as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH3T3 cells)
Loading amount 20 µg
Specification NIH3T3 cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 02 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT1080 cells)
Loading amount 10 µg
Specification HT1080 cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"