ab41037 gave a positive signal in the following whole cell lysates:
Uterus (Human)- normal tissue
HeLa (Human epithelial carcinoma cell line)
Placenta (Mouse) - normal tissue
NIH 3T3 (Mouse embryonic fibroblast cell line)
MEF1 (Mouse embryonic fibroblast cell line)
PC12 (Rat adrenal pheochromocytoma cell line)
ab41037 gave a positive signal in the following tissue lysates:
Brain (Mouse),Liver (Mouse),Testis (Mouse),Spinal Cord (Mouse),Ovary (Mouse),Brain (Rat),Liver (Rat)
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
ab41037 appears to detect the 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.
Use a concentration of 10 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix. The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
The precursor is cleaved by a furin endopeptidase.
Secreted > extracellular space > extracellular matrix.
ab41037 appears to detect at 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.
Western blot - Anti-ADAMTS5 antibody (ab41037)
All lanes : Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution
Lane 1 : Human placenta tissue lysate - total protein (ab29745) Lane 2 :Mouse placenta normal tissue lysate - total protein (ab29139) Lane 3 : Uterus (Human) Whole Cell Lysate - normal tissue Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
ab41037 appears to detect a 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. The doublet observed in human uterus lysate is possibly due to glycosylation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody (ab41037)This image is a courtesy of an anonymous Abreview
ab41037 staining ADAMTS5 in mouse knee joint tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and an enzymatic mediated antigen retrieval step was performed using Proteinase K. Samples were then blocked with 5% serum for 10 minutes at room temperature followed by incubation with the primary antibody at 10µg/ml for 1 hour. An undiluted HRP-conjugated human anti-rabbit monoclonal was used as secondary antibody.
Xu T et al. Hedgehog signal expression in articular cartilage of rat temporomandibular joint and association with adjuvant-induced osteoarthritis. J Oral Pathol Med46:284-291 (2017).
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Qin Y et al. Augmented expression of cardiac ankyrin repeat protein is induced by pemetrexed and a possible marker for the pemetrexed resistance in mesothelioma cells. Cancer Cell Int17:120 (2017).
Read more (PubMed: 29238267) »