Anti-ADAMTS5 antibody - Carboxyterminal end (ab39202)


  • Product nameAnti-ADAMTS5 antibody - Carboxyterminal end
    See all ADAMTS5 primary antibodies
  • Description
    Rabbit polyclonal to ADAMTS5 - Carboxyterminal end
  • Specificityab39202 recognises the Carboxyterminal end of ADAMTS5.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide crresponding to aa 850-890 of the carboxyterminal end of human ADAMTS5.

    (Peptide available as ab43061.)

  • Positive control
    • IHC-P: Human normal liver parenchym



Our Abpromise guarantee covers the use of ab39202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 102 kDa. when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. This antibody recognizes the zymogen of ADAMTS5 at 120 kDa in reduced Western blots, activated forms at 73 kDa (major bands), and breakdown products at 50 kDa and 40 kDa in cell lysates. Dilution optimised using Chromogenic detection.


  • FunctionCleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
  • Tissue specificityExpressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.
  • Sequence similaritiesContains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 2 TSP type-1 domains.
  • DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
    • A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase 2) antibody
    • A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 5 antibody
    • ADAM TS 11 antibody
    • ADAM TS 5 antibody
    • ADAM TS5 antibody
    • ADAM-TS 11 antibody
    • ADAM-TS 5 antibody
    • ADAM-TS5 antibody
    • ADAMTS 11 antibody
    • ADAMTS 5 antibody
    • ADAMTS-11 antibody
    • ADAMTS-5 antibody
    • ADAMTS11 antibody
    • Adamts5 antibody
    • ADMP 2 antibody
    • ADMP-2 antibody
    • ADMP2 antibody
    • Aggrecanase 2 antibody
    • Aggrecanase-2 antibody
    • ATS5_HUMAN antibody
    • FLJ36738 antibody
    • Implantin antibody
    • ThromboSpondin motif-5 antibody
    see all

Anti-ADAMTS5 antibody - Carboxyterminal end images

  • Ab39202 staining Human normal liver parenchym. Staining is localized to cytoplasma and secreted.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-ADAMTS5 antibody - Carboxyterminal end (ab39202)

ab39202 has not yet been referenced specifically in any publications.

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