Overview

  • Product nameAnti-ADAMTS7 antibody
    See all ADAMTS7 primary antibodies
  • Description
    Rabbit polyclonal to ADAMTS7
  • SpecificityThis antibody is specific for the prpetide domain after the second furin cleavage site of the full lenght ADAMTS7 and of the short form ADAMTS7A.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the propeptide domain of human ADAMTS7, after the second furin cleavage site between amino acids 80-140.

    (Peptide available as ab45240.)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab45044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 300kDa (predicted molecular weight: 181 kDa). Glycosylation and the abundance of cysteine residues gives ADAMTS7 a greater apparent molecular weight of around 300kDa on reduced SDS-PAGE gels. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMetalloprotease that may play a role in the degradation of COMP.
    • Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments.
    • Sequence similaritiesContains 1 disintegrin domain.
      Contains 1 peptidase M12B domain.
      Contains 1 PLAC domain.
      Contains 8 TSP type-1 domains.
    • DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      N-glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs. N- and C-glycosylations can also facilitate secretion. O-glycosylated proteoglycan. Contains chondroitin sulfate.
      May be cleaved by a furin endopeptidase (By similarity). The precursor is sequentially processed.
    • Cellular localizationSecreted, extracellular space, extracellular matrix. Also found associated with the external cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein antibody
      • A disintegrin and metalloproteinase with thrombospondin motifs 7 antibody
      • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7 antibody
      • A disintegrin like and metalloprotease with thrombospondin type 1 motif 7 antibody
      • ADAM metallopeptidase with thrombospondin type 1 motif 7 antibody
      • ADAM metallopeptidase with thrombospondin type 1 motif 7 preproprotein antibody
      • ADAM TS 7 antibody
      • ADAM TS7 antibody
      • ADAM-TS 7 antibody
      • ADAM-TS7 antibody
      • ADAMTS 7 antibody
      • ADAMTS-7 antibody
      • Adamts7 antibody
      • ATS7_HUMAN antibody
      • COMPase antibody
      • DKFZp434H204 antibody
      see all

    Anti-ADAMTS7 antibody images

    • All lanes : Anti-ADAMTS7 antibody (ab45044) at 1/1000 dilution

      Lane 1 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA) at 35 µl
      Lane 2 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA) at 30 µl
      Lane 3 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA) at 25 µl


      Predicted band size : 181 kDa

    References for Anti-ADAMTS7 antibody (ab45044)

    This product has been referenced in:
    • Meliopoulos VA  et al. MicroRNA regulation of human protease genes essential for influenza virus replication. PLoS One 7:e37169 (2012). WB ; Human . Read more (PubMed: 22606348) »

    See 1 Publication for this product

    Product Wall

    Gracias por su respuesta. Desafortunadamente, no tenemos lisados de cartilago en nuestro catalogo. Nosotros tenemos un lisado de tumor en hueso, pero no estoy segura si eso contiene condrocitos, este producto es ab29359. Espero que esto sea de alguna a...

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    Gracias por contactarnos. Para ab45041, nosotros recomendamos cerebro (ab30069) como un control positivo. Desafortunadamente, ADAMTS7 solo es expresado en plaquetas y medula óssea y no vendemos esos lisados. Podria ser que encuentres algo usable...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"