ADAMTS7 antibody - Spacer region (ab28557)

Product Name 

ADAMTS7 antibody - Spacer region

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAMTS7 - Spacer region

Immunogen 

Synthetic peptide based on the carboxyterminal end of human ADAMTS7 between amino acids 890-950.

(Peptide available as ab41240.)

Reacts with 
(species key)

Hu

Specificity 

ab28557 recognizes metalloproteinase ADAMTS7.

We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS7 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Predicted molecular weight: 184 kDa. This antibody recognizes the zymogen of ADAMTS7 at around 300 kD in reduced Western blots, activated or alternatively spliced forms at 180-120 kD (major bands) and breakdown products at 58-45 kD in cell lysates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted protein; extracellular space; extracellular matrix.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family

Relevance 

ADAMTS proteases are secreted enzymes containing a prometalloprotease domain of the reprolysin type. The ADAMTS proteases function in processing of procollagens and von Willebrand factor as well as catabolism of aggrecan, versican and brevican. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), the function of ADAMTS7 is still poorly understood. ADAMTS7 most resembles ADAMTS12 in overall structure. ADAMTS7 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Sucrose, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

The antibody has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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