ADAMTS8 antibody - Carboxyterminal end (ab28558)

Product Name 

ADAMTS8 antibody - Carboxyterminal end

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAMTS8 - Carboxyterminal end

Immunogen 

Synthetic peptide based on the carboxyterminal end of human ADAMTS8.(Peptide available as ab4142.)

Reacts with 
(species key)

Hu

Specificity 

ab28558 recognizes metalloproteinase ADAMTS8.

We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS8 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Predicted molecular weight: 97 kDa. This antibody recognizes the zymogen of ADAMTS8 at 110 kD in reduced Western blots, the activated form at 64 kD (major band) and breakdown products at 30 kD of cell lysates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted protein; extracellular space; extracellular matrix.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family

Relevance 

ADAMTS proteases are secreted enzymes containing a prometalloprotease domain of the reprolysin type. The ADAMTS proteases function in processing of procollagens and von Willebrand factor as well as catabolism of aggrecan, versican and brevican. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration.

A member of the metalloproteinase family containing disintegrin like domains (ADAMs), the function of ADAMTS8 is still poorly understood. ADAMTS8 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active on a range of substrates. ADAMTS8 is inhibited by the endogenous MMP inhibitors, TIMP1, 2, 3 and 4, but most efficiently by TIMP3. In addition to the metalloprotease domain, ADAMTS8 has a propeptide domain, a Prohormone Convertase (PC, furin) cleavage site, a cysteine rich domain and thrombospondin 1 like domains.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Sucrose, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

The antibody has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Customer reviews (feedback) regarding ADAMTS8 antibody - Carboxyterminal end

Customer FAQs regarding ADAMTS8 antibody - Carboxyterminal end

Protocols for ADAMTS8 antibody - Carboxyterminal end

Price and availability of products related to ADAMTS8 antibody - Carboxyterminal end

ADAMTS8 antibody - Carboxyterminal end - related products: