Overview

  • Product nameAnti-ADAR1 antibody
    See all ADAR1 primary antibodies
  • Description
    Mouse polyclonal to ADAR1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-1226 of Human ADAR1(P55265)

  • Positive control
    • WB: lysate from liver or HepG2 cells. IHC: lung tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 104 kDa.
IHC-P Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionConverts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression.
  • Tissue specificityUbiquitously expressed, highest levels were found in brain and lung.
  • Involvement in diseaseDefects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.
  • Sequence similaritiesContains 1 A to I editase domain.
    Contains 2 DRADA repeats.
    Contains 3 DRBM (double-stranded RNA-binding) domains.
  • Post-translational
    modifications
    Sumoylation reduces RNA-editing activity.
  • Cellular localizationCytoplasm. Nucleus > nucleolus. Isoform 1 is found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5 is found exclusively in the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 136 kDa double-stranded RNA-binding protein antibody
    • 136kDa double stranded RNA binding protein antibody
    • Adar 1 antibody
    • ADAR antibody
    • Adar1 antibody
    • Adenosine deaminase acting on RNA 1 A antibody
    • Adenosine deaminase RNA specific 1 antibody
    • Adenosine deaminase RNA specific antibody
    • Adenosine deaminase that act on RNA antibody
    • AGS6 antibody
    • AV242451 antibody
    • Double stranded RNA specific adenosine deaminase antibody
    • Double-stranded RNA-specific adenosine deaminase antibody
    • Double-stranded RNA-specific editase Adar antibody
    • DRADA antibody
    • Dsh antibody
    • Dsrad antibody
    • DSRAD_HUMAN antibody
    • dsRNA adenosine deaminase antibody
    • EC 3.5.4.- antibody
    • G1P1 antibody
    • IFI 4 antibody
    • IFI-4 antibody
    • IFI4 antibody
    • Ifi4 protein antibody
    • Interferon induced protein 4 antibody
    • Interferon inducible protein 4 antibody
    • Interferon-inducible protein 4 antibody
    • K88DSRBP antibody
    • mZaADAR antibody
    • P136 antibody
    • Pre-mRNA adenosine deaminase antibody
    • RNA adenosine deaminase 1 antibody
    • RNA-editing deaminase 1 antibody
    • RNA-editing enzyme 1 antibody
    see all

Anti-ADAR1 antibody images

  • Anti-ADAR1 antibody (ab88574) at 1 µg/ml + Human liver lysate at 50 µg

    Predicted band size : 104 kDa
  • Anti-ADAR1 antibody (ab88574) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 104 kDa
  • ab88574 at 3µg/ml staining ADAR1 in formalin-fixed, paraffin-embedded Human lung tissue.
  • Immunofluorescent staining of ADAR1 on HeLa cells using ab88574 at 10 µg/ml.
  • Immunofluorescence analysis of MOCK-infected HEK293T cells, staining ADAR1 (green) with ab88574.

    Cells were fixed in 4% formaldehyde and permeabilized with 0.2% Triton X-100 before incubating with fluorescent-conjugated anti-mouse secondary antibody. Nuclei were stained with DAPI (blue).

References for Anti-ADAR1 antibody (ab88574)

This product has been referenced in:
  • Anadón C  et al. Gene amplification-associated overexpression of the RNA editing enzyme ADAR1 enhances human lung tumorigenesis. Oncogene N/A:N/A (2015). IF ; Human . Read more (PubMed: 26640150) »
  • Cachat A  et al. ADAR1 enhances HTLV-1 and HTLV-2 replication through inhibition of PKR activity. Retrovirology 11:93 (2014). Read more (PubMed: 25389016) »

See all 6 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Breast Cancer Cell Lines (MCF7 & BT549))
Total protein in input 6e+007 cells
Immuno-precipitation step Protein G
Specification Breast Cancer Cell Lines (MCF7 & BT549)
Username

Abcam user community

Verified customer

Submitted Apr 27 2016

The ADAR antibody ab88574 is raised against full-length human ADAR1, and it is a polyclonal antibody, which means it will potentially recognize all ADAR isoforms.

Polyclonal antibodies are composed of many antibodies specific for different epi...

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Thank you for your enquiry.

I am pleased to provide the following IHC-P protocol used for testing ab88574 . As discussed on the telephone, please note that this would be a guideline only and may require some further optimization.

...

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Thank you for contacting Abcam about this issue. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. Before I may issue a free of charge replacement, I would appreciate it if you could sen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"