- Datasheet
- Specific References (9)
- Protocols
Overview
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Product nameAnti-ADAR1 antibody
See all ADAR1 primary antibodies -
DescriptionMouse polyclonal to ADAR1
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Full length protein, corresponding to amino acids 1-1226 of Human ADAR1(P55265)
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Positive control
- WB: lysate from liver or HepG2 cells. IHC: lung tissue. ICC/IF: HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab88574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 104 kDa. | |
| IHC-P | Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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FunctionConverts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression.
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Tissue specificityUbiquitously expressed, highest levels were found in brain and lung.
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Involvement in diseaseDefects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.
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Sequence similaritiesContains 1 A to I editase domain.
Contains 2 DRADA repeats.
Contains 3 DRBM (double-stranded RNA-binding) domains. -
Post-translational
modificationsSumoylation reduces RNA-editing activity. -
Cellular localizationCytoplasm. Nucleus > nucleolus. Isoform 1 is found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5 is found exclusively in the nucleolus.
- Information by UniProt
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Database links
- Entrez Gene: 103 Human
- Omim: 146920 Human
- SwissProt: P55265 Human
- Unigene: 12341 Human
- Unigene: 679967 Human
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Alternative names
- 136 kDa double-stranded RNA-binding protein antibody
- 136kDa double stranded RNA binding protein antibody
- Adar 1 antibody
see all
Images
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Western blot - Anti-ADAR1 antibody (ab88574)Anti-ADAR1 antibody (ab88574) at 1 µg/ml + Human liver lysate at 50 µg
Predicted band size: 104 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-ADAR1 antibody (ab88574)Image from Emmott E et al., J Proteome Res 9:5335-45 (2010). DOI: 10.1021/pr100593g; Fig 4.; October 1, 2010, Journal of Proteome Research with permission from the American Chemical Society.Immunofluorescence analysis of MOCK-infected HEK293T cells, staining ADAR1 (green) with ab88574.
Cells were fixed in 4% formaldehyde and permeabilized with 0.2% Triton X-100 before incubating with fluorescent-conjugated anti-mouse secondary antibody. Nuclei were stained with DAPI (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAR1 antibody (ab88574)ab88574 at 3µg/ml staining ADAR1 in formalin-fixed, paraffin-embedded Human lung tissue. -
Western blot - Anti-ADAR1 antibody (ab88574)Anti-ADAR1 antibody (ab88574) at 1 µg/ml + HepG2 cell lysate at 50 µg
Predicted band size: 104 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-ADAR1 antibody (ab88574)Immunofluorescent staining of ADAR1 on HeLa cells using ab88574 at 10 µg/ml.
Protocols
References
This product has been referenced in:
- Lazzari E et al. Alu-dependent RNA editing of GLI1 promotes malignant regeneration in multiple myeloma. Nat Commun 8:1922 (2017). Read more (PubMed: 29203771) »
- Qi L et al. An RNA editing/dsRNA binding-independent gene regulatory mechanism of ADARs and its clinical implication in cancer. Nucleic Acids Res 45:10436-10451 (2017). Read more (PubMed: 28985428) »
Customer reviews and Q&As
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