The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 104 kDa.
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.
Use a concentration of 10 µg/ml.
Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression.
Ubiquitously expressed, highest levels were found in brain and lung.
Involvement in disease
Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.
Contains 1 A to I editase domain. Contains 2 DRADA repeats. Contains 3 DRBM (double-stranded RNA-binding) domains.
Sumoylation reduces RNA-editing activity.
Cytoplasm. Nucleus > nucleolus. Isoform 1 is found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5 is found exclusively in the nucleolus.
Double-stranded RNA-specific editase Adar antibody
dsRNA adenosine deaminase antibody
EC 3.5.4.- antibody
IFI 4 antibody
Ifi4 protein antibody
Interferon induced protein 4 antibody
Interferon inducible protein 4 antibody
Interferon-inducible protein 4 antibody
Pre-mRNA adenosine deaminase antibody
RNA adenosine deaminase 1 antibody
RNA-editing deaminase 1 antibody
RNA-editing enzyme 1 antibody
Western blot - Anti-ADAR1 antibody (ab88574)
Anti-ADAR1 antibody (ab88574) at 1 µg/ml + Human liver lysate at 50 µg
Predicted band size: 104 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ADAR1 antibody (ab88574)Image from Emmott E et al., J Proteome Res 9:5335-45 (2010). DOI: 10.1021/pr100593g; Fig 4.; October 1, 2010, Journal of Proteome Research with permission from the American Chemical Society.
Immunofluorescence analysis of MOCK-infected HEK293T cells, staining ADAR1 (green) with ab88574.
Cells were fixed in 4% formaldehyde and permeabilized with 0.2% Triton X-100 before incubating with fluorescent-conjugated anti-mouse secondary antibody. Nuclei were stained with DAPI (blue).