Validated using a knockout cell line
Recombinant
RabMAb

Anti-ADAR1 antibody [EPR7033] (ab126745)

Overview

  • Product name
    Anti-ADAR1 antibody [EPR7033]
    See all ADAR1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7033] to ADAR1
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ADAR1 aa 200-300. The exact sequence is proprietary.
    Database link: P55265

  • Positive control
    • HeLa, Ramos and SH-SY5Y cell lysates; Human brain tissue; HeLa cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 136 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression.
    • Tissue specificity
      Ubiquitously expressed, highest levels were found in brain and lung.
    • Involvement in disease
      Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.
    • Sequence similarities
      Contains 1 A to I editase domain.
      Contains 2 DRADA repeats.
      Contains 3 DRBM (double-stranded RNA-binding) domains.
    • Post-translational
      modifications
      Sumoylation reduces RNA-editing activity.
    • Cellular localization
      Cytoplasm. Nucleus > nucleolus. Isoform 1 is found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5 is found exclusively in the nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 136 kDa double-stranded RNA-binding protein antibody
      • 136kDa double stranded RNA binding protein antibody
      • Adar 1 antibody
      • ADAR antibody
      • Adar1 antibody
      • Adenosine deaminase acting on RNA 1 A antibody
      • Adenosine deaminase RNA specific 1 antibody
      • Adenosine deaminase RNA specific antibody
      • Adenosine deaminase that act on RNA antibody
      • AGS6 antibody
      • AV242451 antibody
      • Double stranded RNA specific adenosine deaminase antibody
      • Double-stranded RNA-specific adenosine deaminase antibody
      • Double-stranded RNA-specific editase Adar antibody
      • DRADA antibody
      • Dsh antibody
      • Dsrad antibody
      • DSRAD_HUMAN antibody
      • dsRNA adenosine deaminase antibody
      • EC 3.5.4.- antibody
      • G1P1 antibody
      • IFI 4 antibody
      • IFI-4 antibody
      • IFI4 antibody
      • Ifi4 protein antibody
      • Interferon induced protein 4 antibody
      • Interferon inducible protein 4 antibody
      • Interferon-inducible protein 4 antibody
      • K88DSRBP antibody
      • mZaADAR antibody
      • P136 antibody
      • Pre-mRNA adenosine deaminase antibody
      • RNA adenosine deaminase 1 antibody
      • RNA-editing deaminase 1 antibody
      • RNA-editing enzyme 1 antibody
      see all

    Images



    • Predicted band size : 136 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: ADAR1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HepG2 cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab126745 observed at 130 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab126745 was shown to recognize ADAR1 when ADAR1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ADAR1 knockout samples were subjected to SDS-PAGE. ab126745 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-ADAR1 antibody [EPR7033] (ab126745) at 1/1000 dilution

      Lane 1 : HeLa (treated with IFN-alpha) cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 136 kDa
    • ab126745, at 1/50 dilution, staining ADAR1 in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • ab126745, at 1/50 dilution, staining ADAR1 in Hela cells by Immunofluorescence.
    • Flow cytometric analysis of permeabilized Ramos cells, staining ADAR1 (red) with ab126745.

      1x106 cells were collected and washed with blocking buffer. Cells were fixed with 2% paraformaldehyde, permeabilized with 1X FACS permeabilizing solution and blocked with blocking buffer for 30 minutes at room temperature. Cells were incubated with primary antibody (1/10) for 30 minutes at room temperature before a fluorescently-conjugated secondary antibody or 30 min at room temperature. A rabbit IgG was used as a negative control (green).

    References

    This product has been referenced in:
    • Dou N  et al. Aberrant overexpression of ADAR1 promotes gastric cancer progression by activating mTOR/p70S6K signaling. Oncotarget 7:86161-86173 (2016). Read more (PubMed: 27863387) »
    • Arias C  et al. KSHV 2.0: a comprehensive annotation of the Kaposi's sarcoma-associated herpesvirus genome using next-generation sequencing reveals novel genomic and functional features. PLoS Pathog 10:e1003847 (2014). WB ; Human . Read more (PubMed: 24453964) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Suite à notre conversation téléphonique, j’ai contacté le laboratoire à propos des résultats de cytométrie en flux pour ab126745 et ab126755.

    Les images peuvent être trouvées...

    Read More


    Unfortunately, we do not currently have any publicationfor http://www.abcam.com/ab126745. We will update the on-line datasheet for this product as soon as we receive additional data.

    The recommended protocol can be found via this link: ht...

    Read More

    Thank you for contacting us.

    ab126755 is raised against a peptide from the C-terminal region (within last 50 amino acids) of isoform 1 of human ADAR1.
    ab126745 is raised against a peptide within the range from amino acid 200 to 250. Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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