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Rabbit polyclonal to ADAT1
HumanPredicted to work with:
Mouse, Rat, Cow, Dog
Synthetic peptide: AISWSAVPEQ PLDVTANGFP QGTTKKTIGS LQARSQISKV ELFRSFQKLL, corresponding to amino acids 397-446 of Human ADAT1
- Jurkat cell lysate and Human heart tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use a concentration of 4 - 8 µg/ml.
||Use at an assay dependent concentration.
Specifically deaminates adenosine-37 to inosine in tRNA-Ala.
Belongs to the ADAT1 family.
Contains 1 A to I editase domain.
Information by UniProt
- ADAT 1 antibody
- ADAT1 antibody
- ADAT1_HUMAN antibody
Western blot - ADAT1 antibody (ab50994)
Lane 1 : MW marker
Lane 2 : Anti-ADAT1 antibody (ab50994) at 1.25 µg/ml
Lane 1 : As above
Lane 2 : Jurkat cell lysate at 10 µg
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 39 kDa
Observed band size : 39 kDa
Immunohistochemistry (Paraffin-embedded sections) - ADAT1 antibody (ab50994)
ab50994 staining of Paraffin embeded Human heart tissue in IHC application at 4-8 ug/ml dilution. Cells with positive label (Myocardial cells) are indicated with arrows. Magnification: 400x
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"