Overview

  • Product nameAnti-ADAT1 antibody
    See all ADAT1 primary antibodies
  • Description
    Rabbit polyclonal to ADAT1
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide: AISWSAVPEQ PLDVTANGFP QGTTKKTIGS LQARSQISKV ELFRSFQKLL, corresponding to amino acids 397-446 of Human ADAT1

  • Positive control
    • Jurkat cell lysate and Human heart tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Target

Anti-ADAT1 antibody images

  • Lane 1 : MW marker
    Lane 2 : Anti-ADAT1 antibody (ab50994) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • ab50994 staining of Paraffin embeded Human heart tissue in IHC application at 4-8 ug/ml dilution. Cells with positive label (Myocardial cells) are indicated with arrows. Magnification: 400x

References for Anti-ADAT1 antibody (ab50994)

ab50994 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"