Overview

  • Product name
  • Description
    Rabbit polyclonal to ADAT2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: PKPAASGACS VSAEETEKWM EEAMHMAKEA LENTEVPVGC LMVYNNEVVG KGRNEVNQTK NATRHAEMVA IDQVLDWCRQ SGKSPSEVFE, corresponding to amino acids 7-96 of Human ADAT2.

  • Positive control
    • Human stomach tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab122280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Probably participates in deamination of adenosine-34 to inosine in many tRNAs.
  • Sequence similarities
    Belongs to the cytidine and deoxycytidylate deaminase family. ADAT2 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • adat2 antibody
    • ADAT2_HUMAN antibody
    • Adenosine deaminase tRNA specific 2 antibody
    • Adenosine deaminase tRNA specific 2 TAD2 homolog antibody
    • DEADC1 antibody
    • Deaminase domain containing 1 antibody
    • Deaminase domain containing protein 1 antibody
    • Deaminase domain-containing protein 1 antibody
    • dJ20N2 antibody
    • dJ20N2.1 antibody
    • TAD2 antibody
    • tRNA specific adenosine 34 deaminase subunit ADAT2 antibody
    • tRNA specific adenosine deaminase 2 antibody
    • tRNA specific adenosine deaminase 2 homolog antibody
    • tRNA-specific adenosine deaminase 2 antibody
    • tRNA-specific adenosine-34 deaminase subunit ADAT2 antibody
    see all

Images

  • ab122280, at 1/150 dilution, staining ADAT2 in paraffin-embedded Human stomach tissue by Immunohistochemistry.

References

ab122280 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Caenorhabditis elegans Tissue lysate - whole (whole organism)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
40 µg
Treatment
70c for 10 minutes equal parts sample and 2x Laemmli buffer
Specification
whole organism
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Mar 29 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up