The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/20,000.
WB: 1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). Several extra bands are visible; we have no explanation for the presence of these bands.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThe function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr).
Sequence similaritiesBelongs to the protein kinase superfamily. ADCK protein kinase family. Contains 1 protein kinase domain.
Uncharacterized aarF domain containing protein kinase 5 antibody
Uncharacterized aarF domain-containing protein kinase 5 antibody
Anti-ADCK5 antibody images
Western blot - ADCK5 antibody (ab64978)
All lanes : Anti-ADCK5 antibody (ab64978) at 1/500 dilution
Lane 1 : extracts from HeLa cells (5-30µg total protein) Lane 2 : extracts from 293 cells (5-30µg total protein) Lane 3 : extracts from Huvec cells (5-30µg total protein) Lane 4 : extracts from Huvec cells (5-30µg total protein) with 5-10µg of the immunising peptide
Predicted band size : 66 kDa Observed band size : 66 kDa Additional bands at : 22 kDa,85 kDa. We are unsure as to the identity of these extra bands.
References for Anti-ADCK5 antibody (ab64978)
has not yet been referenced specifically in any publications.
Publishing research using ab64978? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"