Overview

  • Product nameAnti-ADCY7 antibody
    See all ADCY7 primary antibodies
  • Description
    Rabbit polyclonal to ADCY7
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized peptide derived from an internal region of Human ADCY7.

  • Positive control
    • NIH3T3 cells; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab111244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • RelevanceThe membrane-bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein-coupled receptors (GPCRs). Using the high inter-species homology of mammalian AC isoforms, nine Adenylyl cyclase (AC) isoforms, encoded by separate genes, have been identified until today. Human adenylate cyclase genes comprise of 11 to 26 exons, which are distributed over 16 to 430kb. The expression profile of these 9 AC isoforms in a panel of 16 human tissues and in human embryonic kidney (HEK) cells have been demonstrated earlier. The cAMP synthesizing enzymes are found in two forms: cytosolic (soluble) and membrane-bound (particulate). Stimulation of adenylate cyclases produce cAMP form ATP in response to the activation of GPCRs by various hormones, neurotransmitters and other regulatory molecules. cAMP, in subsequent steps down the signal transduction pathway, can stimulate cAMP-dependent protein kinase A (cPKA), and several other target molecules. Activation of cPKA can phosphorylate a broad range of substrates that regulate various metabolic pathways, gene expression, and affect memory functions etc. The stimulation of adenylate cyclases starts with interactions with GPCRs mediated signals initiated by Gs and Gi heterotrimeric G-proteins. The interaction of GPCR agonist (eg. Interaction of isopreternol to beta2 receptors) catalyses the exchange of GDP to GTP that is bound to G proteins. The GTP binding reduces the affinity of GsƒÑ to other GTP binding proteins and Gs-GTP complex stimulate the adenylate cyclase. In last several years, new members of particulate and soluble adenylate cyclase family have been identified and significant progress is made in understanding of the molecular mechanisms that underlie the regulation of these families of enzymes.
  • Cellular localizationIntegral membrane protein.
  • Database links
  • Alternative names
    • adenylate cyclase 7 antibody
    • adenylyl cyclase antibody
    • ATP pyrophosphate-lyase antibody
    • KIAA0037 antibody

Anti-ADCY7 antibody images

  • Immunohistochemistry analysis of paraffin-embedded Human brain tissue, using 1/50 ab111244. The picture on the right is treated with the synthesized peptid
  • Immunofluorescence analysis of NIH-3T3 cells, using 1/100 ab111244. The picture on the right is treated with the synthesized peptide.

References for Anti-ADCY7 antibody (ab111244)

ab111244 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab111244.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"