Overview

  • Product name
    Anti-ADCY9 antibody
    See all ADCY9 primary antibodies
  • Description
    Rabbit polyclonal to ADCY9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Chicken, Guinea pig, Hamster, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset
  • Immunogen

    Synthetic peptide within Human ADCY9 aa 1303-1353 (C terminal). The exact sequence is proprietary. (NP_001107.2)
    Sequence:

    DAHLSPKRPWKEPVKAEERGRFGKAIEKDDCDETGIEEANELTKLNVSKS V


    Database link: O60503

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HeLa, Jurkat, 293T, TCMK1 and NIH3T3 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab191213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 151 kDa.

Target

  • Function
    May play a fundamental role in situations where fine interplay between intracellular calcium and cAMP determines the cellular function. May be a physiologically relevant docking site for calcineurin.
  • Tissue specificity
    Expressed in multiple cells of the lung, with expression highest in airway smooth muscle.
  • Sequence similarities
    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 2 guanylate cyclase domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ADCY 9 antibody
    • ADCY9 antibody
    • ADCY9_HUMAN antibody
    • Adenylate cyclase 9 antibody
    • Adenylate cyclase type 9 antibody
    • Adenylate cyclase type IX antibody
    • Adenylyl cyclase 9 antibody
    • ATP pyrophosphate lyase 9 antibody
    • ATP pyrophosphate-lyase 9 antibody
    • KIAA0520 antibody
    • Type IX adenylyl cyclase antibody
    • Type IX ATP pyrophosphate lyase antibody
    see all

Images

  • Western blot - Anti-ADCY9 antibody (ab191213)
    Western blot - Anti-ADCY9 antibody (ab191213)
    All lanes : Anti-ADCY9 antibody (ab191213) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate in RIPA buffer
    Lane 2 : 293T cell lysate in RIPA buffer
    Lane 3 : Jurkat cell lysate in RIPA buffer
    Lane 4 : TCMK1 cell lysate in NETN buffer
    Lane 5 : NIH3T3 cell lysate in NETN buffer

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 151 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-ADCY9 antibody (ab191213)
    Immunoprecipitation - Anti-ADCY9 antibody (ab191213)

    Immunoprecipitation analysis of HeLa cell lysate (in RIPA buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling ADCY9 using ab191213 at 6 μg per reaction (lane 2). An other rabbit antibpdy against ADCY9 was used in Lane 1. A Control IgG was used in lane 3. For blotting immunoprecipitated ADCY9, ab191213 was used at 0.4 μg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab191213 has not yet been referenced specifically in any publications.

Publishing research using ab191213? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

There are currently no Customer reviews or Questions for ab191213.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up