Recombinant
RabMAb

Anti-ADCY9 [EPR16188] antibody (ab191423)

Overview

  • Product name
    Anti-ADCY9 [EPR16188] antibody
    See all ADCY9 primary antibodies
  • Description
    Rabbit monoclonal [EPR16188] to ADCY9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ADCY9 aa 550-800. The exact sequence is proprietary.
    Database link: O60503

  • Positive control
    • A549, K562, Mouse heart, Mouse spleen, Rat brain, PC12 and NIH 3T3 lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 151 kDa (predicted molecular weight: 151 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      May play a fundamental role in situations where fine interplay between intracellular calcium and cAMP determines the cellular function. May be a physiologically relevant docking site for calcineurin.
    • Tissue specificity
      Expressed in multiple cells of the lung, with expression highest in airway smooth muscle.
    • Sequence similarities
      Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
      Contains 2 guanylate cyclase domains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADCY 9 antibody
      • ADCY9 antibody
      • ADCY9_HUMAN antibody
      • Adenylate cyclase 9 antibody
      • Adenylate cyclase type 9 antibody
      • Adenylate cyclase type IX antibody
      • Adenylyl cyclase 9 antibody
      • ATP pyrophosphate lyase 9 antibody
      • ATP pyrophosphate-lyase 9 antibody
      • KIAA0520 antibody
      • Type IX adenylyl cyclase antibody
      • Type IX ATP pyrophosphate lyase antibody
      see all

    Images

    • All lanes : Anti-ADCY9 [EPR16188] antibody (ab191423) at 1/10000 dilution

      Lane 1 : A549 lysate
      Lane 2 : K562 lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 151 kDa

    • All lanes : Anti-ADCY9 [EPR16188] antibody (ab191423) at 1/2000 dilution

      Lane 1 : Mouse heart lysate
      Lane 2 : Mouse spleen lysate
      Lane 3 : Rat brain lysate
      Lane 4 : PC12 lysate
      Lane 5 : NIH 3T3 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 151 kDa

    References

    ab191423 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    brain
    Blocking step
    5% normal donkey serum, 0.1 TritinX-100, 2% BSA in 0.1M PB 7.4 as blocking agent for 1 hour(s) and 0 minute(s) · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Jana Aradska

    Verified customer

    Submitted Nov 20 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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