Overview

  • Product nameAnti-Adducin 2 antibody
    See all Adducin 2 primary antibodies
  • Description
    Rabbit polyclonal to Adducin 2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 648-697 (VVNGREEEQT AEEILSKGLS QMTTSADTDV DTSKDKTESV TSGPMSPEGS) of Human Adducin 2 (NP_001608).

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMembrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit.
  • Tissue specificityExpressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K562 cells.
  • Sequence similaritiesBelongs to the aldolase class II family. Adducin subfamily.
  • Developmental stageFetal kidney expresses isoforms 3, 4, 5, 6 and 7, and fetal liver expresses isoforms 3 and 4.
  • DomainEach subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationCytoplasm > cytoskeleton. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADD 2 antibody
    • ADD2 antibody
    • ADDB antibody
    • ADDB_HUMAN antibody
    • Adducin 2 (beta) antibody
    • Adducin2 antibody
    • Beta adducin antibody
    • Beta-adducin antibody
    • Erythrocyte adducin subunit beta antibody
    • OTTHUMP00000203378 antibody
    • OTTHUMP00000203908 antibody
    see all

Anti-Adducin 2 antibody images

  • Immunocytochemistry/immunofluorescence analysis of Mouse HEI-OCI auditory cells labeling Adducin 2 with ab84736 at 1:100.
  • Anti-Adducin 2 antibody (ab84736) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 81 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%

References for Anti-Adducin 2 antibody (ab84736)

ab84736 has not yet been referenced specifically in any publications.

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