Overview

  • Product name
    Anti-Adducin antibody
  • Description
    Rabbit polyclonal to Adducin
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Adducin aa 600-650. The exact sequence is proprietary.
    Sequence:

    SPVQSPAKEAETKSPLVSPSKSLEEGTKKTETSKAATTEPETTQPEGVVV N


    Database link: P35612

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab176699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance
    Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
  • Cellular localization
    Cytoplasm › cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • Database links
  • Alternative names
    • ADD1 antibody
    • ADD2 antibody
    • ADD3 antibody
    • ADDA antibody
    • ADDB antibody
    • ADDL antibody
    • Adducin 1 (alpha) antibody
    • Adducin 1 antibody
    • Adducin 2 (beta) antibody
    • Adducin 2 antibody
    • Adducin 3 (gamma) antibody
    • Adducin 3 antibody
    • Adducin like antibody
    • Alpha adducin antibody
    • Beta adducin antibody
    see all

Images

  • All lanes : Anti-Adducin antibody (ab176699) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 81 kDa


    Exposure time : 30 seconds
  • Detection of Adducin by Western Blot of Immunprecipitate.

    Lane 1: ab176699 at 1 µg/ml staining Adducin in 293T whole cell lysate immunoprecipitated using ab176699 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: control IgG.

References

ab176699 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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