The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Catalyzes the exchange of ADP and ATP across the mitochondrial inner membrane.
Involvement in disease
Defects in SLC25A4 are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 2 (PEOA2) [MIM:609283]. Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Adenine Nucleotide Translocase 1 with ab102032 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies of pinealocytes and their processes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Adenine Nucleotide Translocase 1. Right - Merge.
Immunocytochemistry/ Immunofluorescence - Anti-Adenine Nucleotide Translocase 1 antibody (ab102032)This image is courtesy of an anonymous Abreview
ab102032 staining Adenine Nucleotide Translocase 1 from HEK293 cell line from Human by ICC/IF (Immunocytochemistry/immunofluorescence).Cells were fixed with paraformaldehyde,permeabilized with 1% Triton X-100.Samples were incubated with primary antibody for 16 hours at 4 °C.A diluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.