MitoSciences (MSA01-250)

Adenine Nucleotide Translocase (ANT) Immunocapture Kit (ab109713)

Overview

  • Product nameAdenine Nucleotide Translocase (ANT) Immunocapture Kit
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Cow, Human
  • Product overview

    Capture monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture Adenine Nucleotide Translocase from heart mitochondria.

    Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

  • Tested applicationsIPmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab109713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.

Adenine Nucleotide Translocase (ANT) Immunocapture Kit images

  • Adenine Nucleotide Translocase immunoprecipitation using antibody ab109864/MS101c crosslinked to protein G-agarose beads as product ab109713

Protocols

References for Adenine Nucleotide Translocase (ANT) Immunocapture Kit (ab109713)

This product has been referenced in:
  • Duarte FV  et al. Dibenzofuran-induced mitochondrial dysfunction: Interaction with ANT carrier. Toxicol In Vitro 27:2160-2168 (2013). Read more (PubMed: 24008156) »
  • Mailloux RJ  et al. Hexokinase II acts through UCP3 to suppress mitochondrial reactive oxygen species production and maintain aerobic respiration. Biochem J 437:301-11 (2011). Read more (PubMed: 21554247) »

See all 5 Publications for this product

Product Wall

Frozen mitochondria should not be a problem for any immunoprecipitation kits.

Thank you for your reply.

The kit contains 25 uL (settled volume) of beads conjugated to 250 ug of antibody. This is enough to run 5 tests ifyouuse5 uL beads for each test. The 5 uL recommended minimum is for any sample, including mitochondri...

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Yes, the Mitochondria Isolation Kit for Tissue (ab110168) is an ideal kit to use to isolate mitochondria for use with the ANT Immunocapture Kit (ab109713).

For the ANT protocol, "The smallest worki...

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Thank you for contacting us.

ab109864 anti-ANT beads uses the anti-ANT monoclonal antibody ab110322. This antibody is specific to Mouse, Rat, Cow, Human, Caenorhabditis elegans ANT.

The antibody was raised against full length recom...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"