Anti-Adenomatous polyposis coli protein 2 antibody (ab113370)

Overview

  • Product name
    Anti-Adenomatous polyposis coli protein 2 antibody
    See all Adenomatous polyposis coli protein 2 primary antibodies
  • Description
    Rabbit polyclonal to Adenomatous polyposis coli protein 2
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Adenomatous polyposis coli protein 2 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: O95996

  • Positive control
    • Human Brain Cortex and Small Intestine tissues; HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab113370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 244 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC Use at an assay dependent concentration.

Target

  • Function
    Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling.
  • Tissue specificity
    Widely expressed (at protein level). Specifically expressed in the CNS.
  • Sequence similarities
    Belongs to the adenomatous polyposis coli (APC) family.
    Contains 6 ARM repeats.
  • Developmental stage
    Expressed in fetal brain.
  • Cellular localization
    Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasm. Cytoplasm, perinuclear region. Associated with actin filaments (PubMed:11691822). Associated with microtubule network (PubMed:10644998, PubMed:11691822).
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenomatous polyposis coli protein 2 antibody
    • Adenomatous polyposis coli protein-like antibody
    • APC-like antibody
    • apc2 antibody
    • APC2_HUMAN antibody
    • APCL antibody
    see all

Images

  • ab113370, at 5 µg/ml, staining APC2 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry.
  • ab113370, at 5 µg/ml, staining APC2 in Formalin-fixed, Paraffin-embedded Human Small intestine tissue by Immunohistochemistry.
  • Lane 1 : Anti-Adenomatous polyposis coli protein 2 antibody (ab113370) at 1 µg/ml
    Lane 2 : Anti-Adenomatous polyposis coli protein 2 antibody (ab113370) at 2 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate


    Predicted band size : 244 kDa

References

This product has been referenced in:
  • Rashid F  et al. Induction of miR-3648 Upon ER Stress and Its Regulatory Role in Cell Proliferation. Int J Mol Sci 18:N/A (2017). WB ; Human . Read more (PubMed: 28661420) »
  • Hebert AM  et al. Merlin/ERM proteins establish cortical asymmetry and centrosome position. Genes Dev 26:2709-23 (2012). WB ; Human . Read more (PubMed: 23249734) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Small bowel)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH8.5
Permeabilization
No
Specification
Small bowel
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Sep 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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