Anti-Adenomatous polyposis coli protein 2 antibody (ab80018)

Overview

  • Product name
    Anti-Adenomatous polyposis coli protein 2 antibody
    See all Adenomatous polyposis coli protein 2 primary antibodies
  • Description
    Rabbit polyclonal to Adenomatous polyposis coli protein 2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Adenomatous polyposis coli protein 2 aa 2250 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: O95996
    (Peptide available as ab88193)

  • Positive control
    • This antibody gave a positive signal in human spinal cord tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 213 kDa (predicted molecular weight: 243 kDa).

Target

  • Function
    Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling.
  • Tissue specificity
    Widely expressed (at protein level). Specifically expressed in the CNS.
  • Sequence similarities
    Belongs to the adenomatous polyposis coli (APC) family.
    Contains 6 ARM repeats.
  • Developmental stage
    Expressed in fetal brain.
  • Cellular localization
    Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasm. Cytoplasm, perinuclear region. Associated with actin filaments (PubMed:11691822). Associated with microtubule network (PubMed:10644998, PubMed:11691822).
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenomatous polyposis coli protein 2 antibody
    • Adenomatous polyposis coli protein-like antibody
    • APC-like antibody
    • apc2 antibody
    • APC2_HUMAN antibody
    • APCL antibody
    see all

Anti-Adenomatous polyposis coli protein 2 antibody images

  • Anti-Adenomatous polyposis coli protein 2 antibody (ab80018) at 1 µg/ml + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 243 kDa
    Observed band size : 213 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 secondsab80018 detects a strong band at 213 kDa in human spinal cord tissue lysate. This antibody was raised against an immunogen that is predicted to cross react with all known isoforms of adenomatous polyposis coli protein 2 (APC2). APC2 has three isoforms, isoform one has a molecular weight of 243 kDa with isoforms two and three a molecular weight of 213 and 243 respectively(SwissProt data). The band we have observed is thought to represent isoform 2 of APC2 (213 kDa).

References for Anti-Adenomatous polyposis coli protein 2 antibody (ab80018)

ab80018 has not yet been referenced specifically in any publications.

Product Wall

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products. We would be pleased to arrange a replacement product free of charge. I am forwarding these details on to our Distributor’s Team who woul...

Read More

Thank you for contacting us. Anja is currently not in the office, so I had a look at this case. We appreciate the time the customer has spent in the laboratory and understand the concerns. In case they have tried the suggestions made previously, ...

Read More

Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody. The details your customer has kindly provided will ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up