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Rabbit polyclonal to Adenosine A1 Receptor
Synthetic peptide corresponding to Human Adenosine A1 Receptor (C terminal).
Database link: P30542
- WB: HeLa and HEK-293Trap cell lysates.
ICC/IF: SK-N-BE cells.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000. Detects a band of approximately 36.5 kDa (predicted molecular weight: 13,36.5 kDa).
Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Belongs to the G-protein coupled receptor 1 family.
Information by UniProt
- A1AR antibody
- A1R antibody
- AA1R antibody
Immunocytochemistry/ Immunofluorescence - Anti-Adenosine A1 Receptor antibody (ab223377)
4% formaldehyde-fixed SK-N-BE cells stained for Adenosine A1 Receptor (green) using ab223377 at 1/100 dilution in ICC/IF. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223377 (D) Merge.
Western blot - Anti-Adenosine A1 Receptor antibody (ab223377)
All lanes :
Anti-Adenosine A1 Receptor antibody (ab223377) at 1/1000 dilutionLane 1 :
HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysateLane 2 :
HEK-293Trap cell lysate
Lysates/proteins at 15 µg per lane.Secondary
Goat Anti-Rabbit IgG HRP at 1/1000 dilution
Developed using the ECL techniquePredicted band size :
13,36.5 kDaObserved band size :
36.5 kDa (why is the actual band size different from the predicted?
)Exposure time :
Blocking buffer: 5% skimmed milk in TBST.
Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.
has not yet been referenced specifically in any publications.
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