Anti-Adenosine A1 Receptor antibody (ab223377)

Overview

  • Product name
    Anti-Adenosine A1 Receptor antibody
    See all Adenosine A1 Receptor primary antibodies
  • Description
    Rabbit polyclonal to Adenosine A1 Receptor
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Adenosine A1 Receptor (C terminal).
    Database link: P30542

  • Positive control
    • WB: HeLa and HEK-293Trap cell lysates. ICC/IF: SK-N-BE cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 36.5 kDa (predicted molecular weight: 13,36.5 kDa).
ICC/IF 1/100.

Target

  • Function
    Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1AR antibody
    • A1R antibody
    • AA1R antibody
    • AA1R_HUMAN antibody
    • Adenosine receptor A1 antibody
    • ADORA 1 antibody
    • ADORA 1 antibody
    • ADORA1 antibody
    • RDC 7 antibody
    • RDC7 antibody
    • Ri antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for Adenosine A1 Receptor (green) using ab223377 at 1/100 dilution in ICC/IF. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223377 (D) Merge.

  • All lanes : Anti-Adenosine A1 Receptor antibody (ab223377) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293Trap cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG HRP at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 13,36.5 kDa
    Observed band size : 36.5 kDa (why is the actual band size different from the predicted?)


    Exposure time : 6 minutes

    Blocking buffer: 5% skimmed milk in TBST.

    Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.

References

ab223377 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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